anti-Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3) Antibodies

Human Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3) interaction partners

1. Druggable negative allosteric site of P2X3 receptors.

2. conclude that the inter-subunit salt bridge between E112 and R198 of the head and dorsal fin domains, respectively, serves to control the mobility of these domains during agonist-activation of the hP2X3R

3. P2X3 expressions in endometriosis endometrium and endometriotic lesions were significantly higher as compared with control endometrium, and positively correlated with pain.

4. P2X3 receptor expression was up-regulated in neurons from patients with temporal lobe epilepsy.

5. X-ray crystal structures of the human P2X3 receptor in apo/resting, agonist-bound/open-pore, agonist-bound/closed-pore/desensitized and antagonist-bound/closed states

6. there is a relationship between the elevated expression of P2X3 receptor and P2X7 receptor in peripheral blood leukocytes and high serum epinephrine and norepinephrine levels in hyperthyroidism patients.

7. Data show that monoclonal antibodies directed against human P2X3 (12D4) potentiated the slow inactivating current mediated by the heteromeric purinergic receptor hP2X2/3 channel.

8. Data indicate P2X3 purinergic receptors as potential new targets for hepatocellular carcinoma (HCC) therapy.

9. Results demonstrate that the stoichiometry of the heterotrimeric hP2X2/3 receptor is not fixed, but determined by the relative availability of P2X2 and P2X3 subunits

10. Urothelial P2X3 receptors decreased significantly in responders after Lipotoxin instillation, but not after BoNT-A injection.

11. The results from this study demonstrate that there is a significant difference in the expression of the purinergic P2X2, P2X3 and P2X7 receptors in the different histological layers of the human urinary bladder.

12. sensory proteins P2X3 and TRPV1 are in correlation with urothelial differentiation, while P2X5 and TRPV4 have unique expression patterns

13. esophagitis increases mRNA expressions of P2X3 and P2X7 receptors accompanied by up-regulation of TRPV1 and neurotrophic factors (NGF and GDNF).

14. Conformational flexibility of the agonist binding jaw of the human P2X3 receptor is a prerequisite for channel opening

15. TRPV1-, TRPV2-, P2X3-, and parvalbumin-immunoreactivity neurons in the human nodose ganglion innervate the pharynx and epiglottis through the pharyngeal branch and superior laryngeal nerve

16. Data indicate that Markov models are suitable to simulate agonist antagonist interactions at fast desensitizing receptors such as the P2X3R.

17. ATP-gated P2X3 channels in enterochromaffin cells are downregulated in ulcerative colitis.

18. Data indicate that MgATP2- activates P2X1 and P2X3, but not P2X2 and P2X4 receptors.

19. An early and broad expression of P2X3 receptors was found in prenatal dorsal root ganglion neurons.

20. HEK 293 cells are not permissive for stable P2X3 expression afer a significant time-dependent cell loss; often used in a recmbinant expression system, HEK cells represent a limiting permissive environment for P2X3 receptors.

Mouse (Murine) Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3) interaction partners

1. This study demonstrated that the P2X3-immunoreactive nerve endings in the rat tracheal mucosa have unique morphological characteristics, and these endings may be rapidly adapting receptors and/or irritant receptors that are activated by mucosal irritant stimuli.

2. Data suggest that the negative inhibition of P2X3R activity by the BNP/NPR-A pathway results in a decreased P2X3R-mediated excitability of trigeminal neurons in wildetype cultures. In familial hemiplegic migraine type-1 model cultures, however, lack of efficient P2X3Rs downregulation contributes to the neuronal hyperexcitability phenotype.

3. P2X3 receptors on mouse trigeminal ganglion neurons are subjected to contrasting modulation by inhibitory brain natriuretic peptide and facilitatory calcitonin gene-related peptide that both operate via complex intracellular signaling.

4. mouse trigeminal neurons endogenous BNP acts on NPR-A receptors to determine constitutive depression of P2X3 receptor function. Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments

5. may be involved in skeletal pain due to high bone turnover

6. This study observed maltodextrin and fat preference deficits in "taste-blind" P2X2/P2X3 knockout mice.

7. a slow modulatory action by BNP on TRPV1 and P2X3 receptors outlining the role of this peptide as a negative regulator of trigeminal sensory neuron excitability to nociceptive stimuli

8. P2X(3)R overexpression may underlie ectopic mechanical allodynia in the whisker pad skin

9. genetic manipulation of TRPV1 and P2X3 leads to reduction in colorectal mechanosensation peripherally and compensatory changes and/or disinhibition of other channels centrally.

10. up-regulation of P2X3 receptors probably is already maximal and is apparently contributed by basal CGRP and BDNF levels

11. Structure and function of P2RX3 is reviewed, and its role in treatment of pain is discussed. [Review Article]

12. interaction between P2X3/TRPV1 and ERs expression in sensory neurons may represent a novel mechanism that can explain the sex differences in nociception observed in clinical practice.

13. An interaction between P2X3 and membrane-associated ERalpha in primary sensory neurones that may represent a novel mechanism to explain sex differences observed in the clinical presentation of visceral nociceptive syndromes.

14. Transcripts for P2X3 were present in nearly all colon neurons examined. About half of the cells only containing P2X3 transcripts, and zymosan did not change this. The fast current triggered by ATP and alpha,beta-meATP is dependent on P2X3 receptors.

15. CACNA1A mutation conferred a novel molecular phenotype to P2X3 receptors of trigeminal ganglion neurons via CaMKII-dependent activation of calcineurin.

16. chronic esophagitis results in upregulation of P2X(3) and its co-localization with TRPV1 receptor in vagal and spinal afferents.

17. Data suggest that double P2X2/P2X3 purinergic receptor knockout mice are unable to respond to NaCl or the artificial sweetener SC45647 as taste stimuli, mirroring the lack of gustatory nerve responses to these substances.

18. In brief access tests, double P2X2/P2X3 purinergic receptor null mice avoided citric acid and hydrochloric acid at the same concentrations as their wild-type counterparts, indicating that these weak acids activate oropharyngeal chemoreceptors.

19. P2X3-/- DRG neurons lacked rapidly desensitizing response to ATP, and both DRG and nodose neurons from P2X3-null mutant mice no longer responded to alpha,beta-methylene ATP (alphabetameATP).

20. P2X3-mediated responses in dorsal root ganglion neurons were changed in the A/J inbred mouse strain, suggesting that the downregulation of sensory P2X3 might cause low sensitivity to tissue injury pain.

Zebrafish Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3) interaction partners

1. Expressed in overlapping, but unique, patterns of sensory neurons in the developing zebrafish.