anti-RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3 (RBFOX3) Antibodies

RNA-binding protein that regulates alternative splicing events (By similarity).. Additionally we are shipping NEUN Proteins (3) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
RBFOX3 146713 A6NFN3
RBFOX3 52897 Q8BIF2
RBFOX3 287847  
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Top anti-NEUN Antibodies at antibodies-online.com

Showing 10 out of 110 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IF (p), IHC (p), WB Frozen section rat brain labeled with Anti-NeuN Polyclonal Antibody, Unconjugated (ABIN732068) at 1:200 followed by conjugation to the secondary antibody and DAB staining Formalin-fixed and paraffin embedded mouse brain labeled with Anti-NeuN Polyclonal Antibody, Unconjugated (ABIN732068) at 1:200 followed by conjugation to the secondary antibody and DAB staining 100 μL Log in to see 3 to 7 Days
$318.45
Details
Cow Mouse Un-conjugated CyTOF, FACS, ICC, IF, IHC, IHC (fro), IHC (p), WB Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Formalin fixed rat brain stem neurons stained with RBFOX3/NeuN Antibody (1B7) to NeuN in green and counter stained with RBFOX3/NeuN Antibody (1B7), our chicken polyclonal antibody to microtubule associated protein 2 in red. The nuclei of cells are revealed with DAPI in blue. The RBFOX3/NeuN Antibody (1B7) antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of other cell types. The MAP2 antibody binds to dendrites and overlaps with Fox3 staining in perikarya. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells. Note that we have used the same immunogen to generate a rabbit polyclonal antibody to Fox3/NeuN, RPCA-Fox3. Western Blot: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - Western blot of crude rat brain extract stained with NBP1-92693. RBFOX/Neun is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa. 0.1 mL Log in to see 7 to 9 Days
$444.19
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (ff), IHC (fro), IHC (p), WB Immunohistochemistry: RBFOX3/NeuN Antibody [NBP1-77686] - Analysis of NeuN in mouse brain using DAB with hematoxylin counterstain. Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody generated a strong nuclear with weak cytoplasmic staining in NeuN reactive cells. 0.1 mL Log in to see 7 to 9 Days
$485.88
Details
Cow Mouse APC FACS Flow Cytometry: RBFOX3/NeuN Antibody (1B7)  - An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693APC (blue) and a matched isotype control NB600-986APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Flow Cytometry: RBFOX3/NeuN Antibody (1B7)  - An intracellular stain was performed on U87MG cells with RBFOX3/NeuN antibody (1B7) NBP1-92693APC (blue) and a matched isotype control APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. 0.1 mL Log in to see 8 to 11 Days
$556.31
Details
Cow Rabbit Un-conjugated ICC, IF, IHC (fro), SimWes, WB Immunohistochemistry-Frozen: RBFOX/Neun Antibody [NBP1-92716] - Paraformaldehyde fixed frozen section of adult rat cortex stained with NBP1-92716 in red and counterstained for DNA in blue. Note that NBP1-92716 stains neuronal nuclei and distal perikarya. These antibodies do not bind to the nuclei of perikarya of non-neuronal cells, so that they can be used to identify neurons. Simple Western: RBFOX3/NeuN Antibody [NBP1-92716] - Simple Western lane view shows a specific band for RBFOX3/NeuN in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. 0.1 mL Log in to see 7 to 9 Days
$444.19
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-89821] - Staining of human cell line A549 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green. Western Blot: RBFOX3/NeuN Antibody [NBP1-89821] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.Lane 2: Human cell line RT-4.Lane 3: Human cell line U-251MG sp 0.1 mL Log in to see 7 to 9 Days
$491.63
Details
Human Rabbit Un-conjugated IHC, WB Western blot analysis of extracts of various cells, using NeuN(RBFOX3) antibody. 100 μL Log in to see 16 Days
$334.02
Details
Cow Mouse PE FACS Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [PE] [NBP1-92693PE] - An intracellular stain was performed on SH-SY5Y cells with RBFOX3 (1B7) antibody NBP1-92693PE (blue) and a matched isotype control ABIN4349554PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. 0.1 mL Log in to see 7 to 9 Days
$556.31
Details
Cow Mouse Alexa Fluor 700 FACS, ICC, IF, IHC (fro), IHC (p), WB Flow Cytometry: RBFOX3/NeuN Antibody (1B7) [Alexa Fluor (R) 700] [NBP1-92693AF700] - FACS analysis of mouse brain cells - Trypsin-EDTA digestion, 2% PFA fixation, primary used at 1:10 dilution/ 1 hour RT incubation (Image provided by Dr Xingrao Ke from Dept. of Pediatrics at Medical College of Wisconsin; see the product review for methodology details). Flow Cytometry: RBFOX3/NeuN Antibody (1B7)   - An intracellular stain was performed on U87-MG cells with RBFOXP3/NeuN (1B7) antibody  (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. 0.1 mL Log in to see 8 to 11 Days
$556.31
Details
Cow Mouse PerCP ICC, IF, WB Flow Cytometry: RBFOX3/NeuN Antibody (1B7)  - An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693PCP (blue) and a matched isotype control NB600-986PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-protein. 0.1 mL Log in to see 8 to 11 Days
$547.69
Details

RBFOX3 Antibodies by Reactivity, Application, Clonality and Conjugate

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Top referenced anti-NEUN Antibodies

  1. Human Polyclonal NEUN Primary Antibody for IF (p), IHC (p) - ABIN732068 : Jiang, Tian, Fu, Zhu, Hou: Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury. in European journal of pharmacology 2010 (PubMed)
    Show all 13 Pubmed References

  2. Human Polyclonal NEUN Primary Antibody for ICC, IF - ABIN4349539 : Sámano, Nasrabady, Nistri: A study of the potential neuroprotective effect of riluzole on locomotor networks of the neonatal rat spinal cord in vitro damaged by excitotoxicity. in Neuroscience 2012 (PubMed)
    Show all 9 Pubmed References

  3. Cow (Bovine) Monoclonal NEUN Primary Antibody for CyTOF, FACS - ABIN4349542 : Morfini, Bosco, Brown, Gatto, Kaminska, Song, Molla, Baker, Marangoni, Berth, Tavassoli, Bagnato, Tiwari, Hayward, Pigino, Watterson, Huang, Banker, Brown, Brady: Inhibition of fast axonal transport by pathogenic SOD1 involves activation of p38 MAP kinase. in PLoS ONE 2013 (PubMed)
    Show all 4 Pubmed References

  4. Human Polyclonal NEUN Primary Antibody for ICC, IF - ABIN4349540 : Bergmann, Liebl, Bernard, Alkass, Yeung, Steier, Kutschera, Johnson, Landén, Druid, Spalding, Frisén: The age of olfactory bulb neurons in humans. in Neuron 2012 (PubMed)
    Show all 2 Pubmed References

  5. Human Polyclonal NEUN Primary Antibody for ICC, IF - ABIN4349562 : Robinson, Chapani, Styan, Vaidyanathan, Willerth: Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells. in Stem cell reviews 2016 (PubMed)

  6. Cow (Bovine) Polyclonal NEUN Primary Antibody for ICC, IF - ABIN4349544 : Zhang, Wang, Tang, Dong, Chan, Sareddy, Vadlamudi, Brann: Brain-derived estrogen exerts anti-inflammatory and neuroprotective actions in the rat hippocampus. in Molecular and cellular endocrinology 2014 (PubMed)

  7. Cow (Bovine) Monoclonal NEUN Primary Antibody for FACS - ABIN4349556 : Cohen, Ke, Liu, Fu, Majnik, Lane: Adverse early life environment increases hippocampal microglia abundance in conjunction with decreased neural stem cells in juvenile mice. in International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2016 (PubMed)

  8. Human Polyclonal NEUN Primary Antibody for IF (p) - ABIN908077 : Fontes, Ramsey, Polk, Koop, Denisova, Belousov: Death of Neurons following Injury Requires Conductive Neuronal Gap Junction Channels but Not a Specific Connexin. in PLoS ONE 2015 (PubMed)

  9. Human Polyclonal NEUN Primary Antibody for IF (p) - ABIN732072 : Song, Gaudin, King, Seo, Suh, Deng, Cui, Tietjen, Huttner, Saltzman: Surface chemistry governs cellular tropism of nanoparticles in the brain. in Nature communications 2017 (PubMed)

  10. Human Polyclonal NEUN Primary Antibody for IF (p) - ABIN732074 : Ridder, Sevko, Heide, Dams, Rupp, Macas, Starmann, Tjwa, Plate, Sültmann, Altevogt, Umansky, Momma: Extracellular vesicle-mediated transfer of functional RNA in the tumor microenvironment. in Oncoimmunology 2015 (PubMed)

More Antibodies against NEUN Interaction Partners

Human RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3 (RBFOX3) interaction partners

  1. RBFOX3 knockdown synergized with 5-FU to inhibit the growth and invasion of HCC (show FAM126A Antibodies) cells through PI3K (show PIK3CA Antibodies)/AKT (show AKT1 Antibodies) and epithelial-mesenchymal transition (EMT (show ITK Antibodies)) signaling, and promote apoptosis by activating the cytochrome-c (show CYCS Antibodies)/caspase (show CASP3 Antibodies) signaling pathway.

  2. In human lung tissue, Claudin-1 (show CLDN7 Antibodies) is higher in RBFOX3-positive cells than in RBFOX3-negative cells. Immunostaining and mRNA quantification revealed that protein levels, but not mRNA levels, of Claudin-1 (show CLDN7 Antibodies) are increased by RBFOX3.

  3. SNPs (rs9900428, rs9907432 and rs7211029) in RBFOX3 associate with sleep latency.

  4. this study show that TGF-beta (show TGFB1 Antibodies)-induced RBFOX3 inhibition plays an important role in epithelial-mesenchymal transition and lung cancer progression

  5. NeuN was recently eventually identified as an epitope of Rbfox3. NeuN is highly conserved among species and is stably expressed during specific stages of development. NeuN has been considered to be a reliable marker of mature neurons, however, this role has been challenged by recent studies indicating that NeuN staining is variable and even absent during certain diseases and states. Review.

  6. RBFOX proteins can facilitate the splicing of micro-exons. We also found that PTBP1 (show PTBP1 Antibodies) likely regulates the inclusion of micro-exons, possibly by repressing the inclusion of micro-exons that are enhanced by RBFOX proteins and other splicing factors.[RBFOX]

  7. RNA splicing occurs in the nucleus hence, the altered localization of RbFox3 to the cytoplasm may lead to the downregulation of neuronal gene expression.

  8. Exome sequencing of 242 rolandic epilepsy patients revealed two novel probably deleterious variants in RBFOX1 (show A2BP1 Antibodies), a frameshift mutation (p.A233Vfs*74) and a hexanucleotide deletion (p.A299_A300del), and a novel nonsense mutation in RBFOX3 (p.Y287*).

  9. High NeuN levels are associated with supratentorial ependymomas.

  10. NeuN immunocytochemical reaction in neurons of human substantia nigra was expressed much weaker than in the nucleus rubrum neurons located in the same sections.

Mouse (Murine) RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3 (RBFOX3) interaction partners

  1. Rbfox3-/- mice displayed normal non-image and image forming functions. Taken together, our results suggest RBFOX3 is dispensable for visual function

  2. Results demonstrate the important role of RBFOX3 during neural development and maturation and show that abnormalities in synaptic structure and function occur in Rbfox3 homozygous knockout mice. These findings may offer mechanistic explanations for human brain diseases associated with dysfunctional RBFOX3.

  3. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function.

  4. In vitro analyses revealed that Rbfox3 functions as a positive and a negative regulator at the stage of pri-miRNA processing to precursor miRNA (pre-miRNA).

  5. Rbfox3-dependent Numb (show NUMB Antibodies) alternative splicing plays an important role in the progression of neuronal differentiation during vertebrate development.

  6. the negative regulation of Rbfox2 (show RBM9 Antibodies) by Rbfox3 through a novel mechanism

  7. During the development of olfactory bulb and epithelium, NeuN is expressed only in mature neural cells migrating from margin area to the inner granule cells layer of olfactory bulb.

  8. NeuN is the Fox-3 gene product and its expression is restricted to neural tissues.

  9. Enzymatic dephosphorylation abolishes NeuN immunoreactivity, confirming that NeuN is indeed a phosphoprotein, and establishing that binding of the defining antibody depends on its state of phosphorylation.

  10. Data show that inhibition of ZNF238 (RP58 (show ZNF238 Antibodies)) expression in mouse GNPs decreases the expression of the neuronal differentiation markers MAP2 and NeuN and downregulates the expression of the cell cycle arrest protein p27 (show CDKN1B Antibodies), a regulator of GNP differentiation.

NEUN (RBFOX3) Antigen Profile

Protein Summary

RNA-binding protein that regulates alternative splicing events (By similarity).

Gene names and symbols associated with RBFOX3

  • RNA binding fox-1 homolog 3 (RBFOX3) antibody
  • RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3) antibody
  • RNA binding protein, fox-1 homolog (C. elegans) 3 (RBFOX3) antibody
  • RNA binding protein, fox-1 homolog 3 (Rbfox3) antibody
  • Fox-3 antibody
  • FOX3 antibody
  • Hrnbp3 antibody
  • Neun antibody
  • Neuna60 antibody
  • RGD1560070 antibody

Protein level used designations for RBFOX3

RNA binding protein fox-1 homolog 3 , fox-1 homolog C , hexaribonucleotide binding protein 3 , neuronal nuclei , neuronal nuclear antigen A60 , RNA binding protein, fox-1 homolog 3

GENE ID SPECIES
146713 Homo sapiens
52897 Mus musculus
511773 Bos taurus
287847 Rattus norvegicus
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