NeuN antibody (AA 51-150)

Catalog No. ABIN732068

Product Details anti-NeuN Antibody

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Binding Specificity: AA 51-150

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NeuN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NeuN

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NeuN antibody (ABIN732068)

Kopec, Rivera, Lacagnina, Hanamsagar, Bilbo: "Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue." in: Journal of neuroscience methods, Vol. 280, pp. 64-76, (2017) (PubMed).

Wang, Tian, Wang, Wei, Wu, Wang, Wang, Yang, Ji, Li: "Anti-inflammatory and retinal protective effects of capsaicin on ischaemia-induced injuries through the release of endogenous somatostatin." in: Clinical and experimental pharmacology & physiology, Vol. 44, Issue 7, pp. 803-814, (2017) (PubMed).

Ridder, Sevko, Heide, Dams, Rupp, Macas, Starmann, Tjwa, Plate, Sültmann, Altevogt, Umansky, Momma: "Extracellular vesicle-mediated transfer of functional RNA in the tumor microenvironment." in: Oncoimmunology, Vol. 4, Issue 6, pp. e1008371, (2015) (PubMed).

Fontes, Ramsey, Polk, Koop, Denisova, Belousov: "Death of Neurons following Injury Requires Conductive Neuronal Gap Junction Channels but Not a Specific Connexin." in: PLoS ONE, Vol. 10, Issue 5, pp. e0125395, (2015) (PubMed).

Hu, Lin, Zhu, Li, Chen: "Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. [Corrected]." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 3998, (2015) (PubMed).

Guo, Zhang, Xu, Zhi, Han, Zhou, Liu, Lin, Zhang: "Bone marrow-derived, neural-like cells have the characteristics of neurons to protect the peripheral nerve in microenvironment." in: Stem cells international, Vol. 2015, pp. 941625, (2015) (PubMed).

Zhao, Dumanis, Tamboli, Rodriguez, Jo Ladu, Moussa, William Rebeck: "Human APOE genotype affects intraneuronal A?1-42 accumulation in a lentiviral gene transfer model." in: Human molecular genetics, Vol. 23, Issue 5, pp. 1365-75, (2014) (PubMed).

Zhao, Zhang, Davis, Rebeck: "Aging reduces glial uptake and promotes extracellular accumulation of A? from a lentiviral vector." in: Frontiers in aging neuroscience, Vol. 6, pp. 210, (2014) (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, (2013) (PubMed).

Li, Liang, Guan, Fan, Chi, Yang: "Regular treadmill running improves spatial learning and memory performance in young mice through increased hippocampal neurogenesis and decreased stress." in: Brain research, Vol. 1531, pp. 1-8, (2013) (PubMed).

Wei, Chen, Chen, Lai, Liu, Deng, Zhou, Zhang, Li, Lii, Liu, Wang, Nie: "β-Asarone inhibits neuronal apoptosis via the CaMKII/CREB/Bcl-2 signaling pathway in an in vitro model and AβPP/PS1 mice." in: Journal of Alzheimer's disease : JAD, Vol. 33, Issue 3, pp. 863-80, (2013) (PubMed).

Zhao, Zhang, Wang, Chen, Ji, Zhang: "(S)-ZJM-289, a nitric oxide-releasing derivative of 3-n-butylphthalide, protects against ischemic neuronal injury by attenuating mitochondrial dysfunction and associated cell death." in: Neurochemistry international, Vol. 60, Issue 2, pp. 134-44, (2012) (PubMed).

Jiang, Fu, Tian, Zhu, Hou: "Curculigoside A attenuates experimental cerebral ischemia injury in vitro and vivo." in: Neuroscience, Vol. 192, pp. 572-9, (2011) (PubMed).

Jiang, Tian, Fu, Zhu, Hou: "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." in: European journal of pharmacology, Vol. 640, Issue 1-3, pp. 75-81, (2010) (PubMed).

Target Details for NeuN

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Alternative Name: FOX3/NeuN (RBFOX3 Products)

Synonyms: FOX-3 antibody, FOX3 antibody, HRNBP3 antibody, NEUN antibody, Fox-3 antibody, Hrnbp3 antibody, NeuN antibody, Neuna60 antibody, Neun antibody, RGD1560070 antibody, RNA binding fox-1 homolog 3 antibody, RNA binding protein, fox-1 homolog (C. elegans) 3 antibody, RNA binding protein, fox-1 homolog 3 antibody, RBFOX3 antibody, Rbfox3 antibody

Background:

Synonyms: FOX3, NEUN, FOX-3, HRNBP3, RNA binding protein fox-1 homolog 3, Fox-1 homolog C, RBFOX3

Background: Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.

Gene ID: 146713

UniProt: A6NFN3