anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) Antibodies

TOP2B encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. Additionally we are shipping Topoisomerase (DNA) II beta 180kDa Kits (15) and Topoisomerase (DNA) II beta 180kDa Proteins (6) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
TOP2B 21974 Q64511
TOP2B 361100  
TOP2B 7155 Q02880
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Top anti-Topoisomerase (DNA) II beta 180kDa Antibodies at antibodies-online.com

Showing 10 out of 67 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-TOP2B Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:1562500  Positive Control:  Jurkat cell lysate TOP2B is supported by BioGPS gene expression data to be expressed in Jurkat WB Suggested Anti-TOP2B  Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500  Positive Control: Jurkat cell lysate  TOP2B is supported by BioGPS gene expression data to be expressed in Jurkat 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP, WB Western Blot and Immunoprecipitation: Topo II Beta Antibody [ABIN251526] - Detection of Human Topo II Beta by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) and 293T (T, 50 mcg) cells. Antibodies: Affinity purified rabbit anti- Topo II Beta antibody ABIN251526 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). Topo II Beta was also immunoprecipitated by another rabbit anti-Topo II Beta antibody and  ABIN251525, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B). Immunohistochemistry-Paraffin: TOP2B Antibody [ABIN2515262] - FFPE section of human small cell lung cancer. Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:250. 0.1 mL Log in to see 7 to 9 Days
$559.35
Details
Dog Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of Topoisomerase 2 beta staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated IHC, ELISA, WB Western blot analysis of extracts from Jurkat cells, using TOP2B Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TOP2B Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), SimWes, WB Immunocytochemistry/Immunofluorescence: TOP2B Antibody  - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Immunohistochemistry-Paraffin: TOP2B Antibody  - Staining of human pancreas shows moderate nuclear positivity. 0.1 mL Log in to see 7 to 9 Days
$494.38
Details
Mouse Rabbit Un-conjugated ICC, IHC, WB Western Blot; Sample: Human K562 cell lysate; Primary Ab: 2µg/mL Rabbit Anti-Mouse TOP2b Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) 100 μg Log in to see 13 to 16 Days
$360.00
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, IHC, WB ABIN6266664 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. ABIN6266664 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody. 100 μL Log in to see 11 to 12 Days
$390.77
Details
Human Rabbit Un-conjugated IP, IHC (p), WB IP Image Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 μg of Topoisomerase II beta antibody , 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody , diluted at 1:1000. EasyBlot anti-rabbit IgG  was used as a secondary reagent. WB Image Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE antibody diluted at 1:3000 100 μL Log in to see 3 to 4 Days
$466.18
Details
Human Rabbit Un-conjugated IHC, IHC (p), IP, WB Immunoprecipitation: TOP2B Antibody  - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 ug of Topoisomerase II beta antibody 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent. 0.1 mL Log in to see 8 to 11 Days
$516.98
Details
Bat Rabbit Un-conjugated WB 50 μg Log in to see 11 to 14 Days
$551.83
Details

Top referenced anti-Topoisomerase (DNA) II beta 180kDa Antibodies

  1. Human Polyclonal TOP2B Primary Antibody for ICC, IF - ABIN251526 : Elliott, Crawford, Mulligan, Summerfield, Newton, Wallis, Mainou-Fowler, Evans, Bedwell, Durkacz, Willmore: Mitoxantrone in combination with an inhibitor of DNA-dependent protein kinase: a potential therapy for high risk B-cell chronic lymphocytic leukaemia. in British journal of haematology 2010 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal TOP2B Primary Antibody for IHC (p), IHC - ABIN257919 : Barajon, Serrao, Arnaboldi, Opizzi, Ripamonti, Balsari, Rumio: Toll-like receptors 3, 4, and 7 are expressed in the enteric nervous system and dorsal root ganglia. in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 (PubMed)

  3. Human Polyclonal TOP2B Primary Antibody for ICC, IF - ABIN4361378 : Aviner, Shenoy, Elroy-Stein, Geiger: Uncovering Hidden Layers of Cell Cycle Regulation through Integrative Multi-omic Analysis. in PLoS genetics 2015 (PubMed)

More Antibodies against Topoisomerase (DNA) II beta 180kDa Interaction Partners

Mouse (Murine) Topoisomerase (DNA) II beta 180kDa (TOP2B) interaction partners

  1. Top2beta controls spinal motor neurons (MN) migration and connectivity; is not required for MN generation or survival; has a selective role in columnar specification. In absence of Top2beta, phrenic MN identity is eroded, while other motor columns are partially preserved but fail to cluster to their proper position. In Top2beta-/- mice, peripheral connectivity is impaired as MNs exhibit a profound deficit in terminal b...

  2. our data establish a combinatorial cell-autonomous and non-cell-autonomous role for Top2b in the late stage of photoreceptor differentiation and maturation

  3. We demonstrated that Nup98-TopIIbeta and Nup98-SETBP1 negatively regulate the XPO1-mediated protein export. Our results will contribute to the understanding of the molecular mechanism by which the Nup98-fusion proteins induce tumorigenesis.

  4. TOP2beta as a factor involved in regulating granulosa cell genomic integrity and follicle atresia.

  5. Results identified topoisomerase IIbeta (Top2beta) as a novel determinant for camptothecin sensitivity.

  6. Top IIbeta might be a down-stream target of Nurr1, which might influence the processes of axon genesis in dopaminergic neurons via the regulation of TOP IIbeta expression.

  7. Findings suggest a chromatin-based targeting of Top2beta to regulatory regions in the genome to govern the transcriptional program associated with neuronal differentiation and longevity.

  8. Data suggest that activity of TOP2B in elongating spermatids is strongly inhibited by PARP1 and PARP2, and this is in turn counteracted by the PAR-degrading activity of PAR glycohydrolase.

  9. proteasomal degradation of TOP2beta induced by the TOP2-DNA covalent complex or the TOP2 circular clamp is due to transcriptional arrest but not DNA damage

  10. DNA topoisomerase II beta deficiency severely affects cerebral cortex stratification: no subplate is discernible, and neurons born at later stages of corticogenesis fail to migrate to the superficial layers.

  11. A nuclear matrix-associated topoisomerase IIB (TOP2B) interacting with an extracellular Mn2+/Ca2+-dependent nuclease cleaves sperm DNA into loop-sized fragments.

  12. Results support a role of topoisomerase IIbeta in activation/repression of developmentally regulated genes at late stages of neuronal differentiation.

  13. These results suggest that the defect in establishing neuromuscular junctions in top2beta knockout mice could be due to the lack of TopIIbeta-mediated neurite outgrowth.

  14. VP-16-induced carcinogenesis involves mainly the beta rather than the alpha isozyme of Top2; VP-16-induced DNA sequence rearrangements and double-strand breaks are found to be Top2beta-dependent

  15. NRF-1 can also directly interact with poly(ADP-ribose) polymerase 1 (PARP-1) and co-purify the PARP-1.DNA-PK.Ku80.Ku70.topoisomerase IIbeta-containing protein complex.

Human Topoisomerase (DNA) II beta 180kDa (TOP2B) interaction partners

  1. our data primarily indicate that topoIIbeta can be used to estimate neuronal differentiation in medulloblastoma

  2. The article discusses origin of topoisomerase II beta, its structure, activities reported in vitro and in vivo along with implications in cellular processes namely transcription, DNA repair, neuronal development, aging, HIV-infection and cancer. (Review)

  3. recent data point to a critical role of topoisomerase II beta (TOP2B), which is a primary target of anthracycline poisoning, in the pathophysiology of anthracycline-induced congestive heart failure .

  4. The decatenation checkpoint is regulated, not only by topo IIalpha, as previously reported, but also by topo IIbeta. The decatenation checkpoint is most efficient when both isoforms are present. Deletion of most of the C-terminus of topo IIbeta, while preserving the nuclear localization signal, and mutation of Y656 in topo IIbeta inhibits the decatenation checkpoint and sensitivity to topo II-targeted drugs.

  5. These results explain why hTOPIIa and hTOPIIa are differentially affected by various poisons and demonstrate the utility of C. elegans in understanding the genetics of drug responses.

  6. TOP2B is positioned to solve topological problems at diverse cis-regulatory elements and its occupancy is a highly ordered and prevalent feature of CTCF/cohesin binding sites that flank TADs.

  7. Data indicate that cortex involvement, lower World Health Organization grade and DNA topoisomerase II positivity were strong predictors for preoperative epileptic seizures.

  8. during early development, TOP2A is likely to have a role in cell proliferation, whereas TOP2B is expressed in post-mitotic cells and may be important in controlling expression of long genes even at this early stage.

  9. TopoIIbeta is involved in the cascade of coactivator complexes that are recruited to LTR for regulation of HIV-1 transcription.

  10. Cinobufacini inhibited the proliferation of the HepG-2 cells induced apoptosis in a dose- and time-dependent manner and downregulated the mRNA and protein expression levels of TOPO I and TOPO II

  11. A direct interaction between Ku70/86 and BRG1 brings together SWI/SNF remodeling capabilities and TOP2beta activity to enhance the transcriptional response to hormone stimulation.

  12. Human cytomegalovirus IE1 exon 4 interacts with Topoisomerase IIbeta (TOPOIIbeta), whose activity is required for viral genome persistence and maintenance via binding to a cis-acting viral maintenance element.

  13. This study demonstrated that top IIbeta overexpression is necessary for RA-induced neuronal differentiation.

  14. Data suggest that DNA topoisomerase IIbeta (topoIIbeta) silencing can significantly alter the gene expression pattern of genes involved in variety of biological processes and signal transduction pathways.

  15. Proteolytic degradation of Top2 enables the processing of Top2.DNA and Top2.RNA covalent complexes by TDP2.

  16. There is an association between chemosensitivity and Pgp, GST-pi and Topo II expression in gastric cancer.

  17. These results are consistent with the hypothesis that etoposide quinone contributes to etoposide-related leukemogenesis through an interaction with topoisomerase IIbeta.

  18. Downregulating topoisomerase IIbeta confers resistance specifically to mitoxantrone.

  19. Top2beta-DNA cleavage complexes arrest transcription elongation and induce a proteasomal degradation of Top2beta on DNA.

  20. results suggest that the low expression of TOPOIIbeta in patients with tongue carcinoma indicates that intrinsic drug resistance may exist in tongue carcinoma, and is associated with tumor differentiation and cisplatin resistance in tongue carcinoma.

Topoisomerase (DNA) II beta 180kDa (TOP2B) Antigen Profile

Protein Summary

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants\; however, the second variant has not yet been fully described.

Gene names and symbols associated with TOP2B

  • DNA topoisomerase II beta (TOP2B) antibody
  • topoisomerase (DNA) II beta (Top2b) antibody
  • DNA topoisomerase II beta (Top2b) antibody
  • microRNA 4442 (MIR4442) antibody
  • D230016L12Rik antibody
  • Top-2 antibody
  • TOP2B antibody
  • top2beta antibody
  • TOPIIB antibody

Protein level used designations for TOP2B

topoisomerase (DNA) II beta 180kDa , DNA topoisomerase II, beta isozyme , DNA topoisomerase 2-beta , topoisomerase II beta , DNA topoisomerase II beta , DNA topoisomerase II, 180 kD , U937 associated antigen , antigen MLAA-44 , topo II beta , topoisomerase IIb , DNA topoisomeraseII_beta , topoisomerase II beta-2

GENE ID SPECIES
460232 Pan troglodytes
21974 Mus musculus
361100 Rattus norvegicus
7155 Homo sapiens
395966 Gallus gallus
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