anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) Antibodies

TOP2B encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. Additionally we are shipping Topoisomerase (DNA) II beta 180kDa Kits (14) and Topoisomerase (DNA) II beta 180kDa Proteins (5) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
TOP2B 21974 Q64511
TOP2B 361100  
TOP2B 7155 Q02880
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Top anti-Topoisomerase (DNA) II beta 180kDa Antibodies at

Showing 10 out of 61 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB <b>WB Suggested Anti-TOP2B Antibody Titration: </b>0.2-1 ug/ml<br><b>ELISA Titer: </b>1:1562500<br><b>Positive Control: </b>Jurkat cell lysate WB Suggested Anti-TOP2B <br /> Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500 <br /> Positive Control: Jurkat cell lysate <br /> TOP2B is supported by BioGPS gene expression data to be expressed in Jurkat 100 μL Log in to see 2 to 3 Days
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP, WB Western Blot and Immunoprecipitation: Topo II Beta Antibody [ABIN251526] - Detection of Human Topo II Beta by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) and 293T (T, 50 mcg) cells. Antibodies: Affinity purified rabbit anti- Topo II Beta antibody ABIN251526 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). Topo II Beta was also immunoprecipitated by another rabbit anti-Topo II Beta antibody and  ABIN251525, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B). Immunohistochemistry-Paraffin: TOP2B Antibody [ABIN2515262] - FFPE section of human small cell lung cancer. Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:250. 0.1 mL Log in to see 7 to 9 Days
Human Rabbit Un-conjugated IHC, ELISA, WB Western blot analysis of extracts from Jurkat cells, using TOP2B Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TOP2B Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), SimWes, WB Immunocytochemistry/Immunofluorescence: TOP2B Antibody  - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Immunohistochemistry-Paraffin: TOP2B Antibody  - Staining of human pancreas shows moderate nuclear positivity. 0.1 mL Log in to see 10 to 13 Days
Human Mouse Un-conjugated BI, IF, WB Western blot analysis of Topo IIbeta on a Jurkat lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Topo IIbeta antibody. Immunofluorescent staining of A549 cells. Cells were seeded in a 96 well imaging plate at ~ 10 000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol and the anti-Topisomerase IIbeta antibody. The second step reagent was FITC goat anti mouse Ig. The image was taken on a Pathway 850 imager using a 20x objective. This antibody also stained HeLa and U2OS cells and can be used with either fix/perm protocol. 50 μg Log in to see 6 to 8 Days
Mouse Rabbit Un-conjugated ICC, IHC, WB Western Blot; Sample: Human K562 cell lysate; Primary Ab: 2µg/mL Rabbit Anti-Mouse TOP2b Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) 100 μg Log in to see 13 to 16 Days
Human Rabbit Un-conjugated IP, IHC (p), WB IP Image Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 μg of Topoisomerase II beta antibody , 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody , diluted at 1:1000. EasyBlot anti-rabbit IgG  was used as a secondary reagent. WB Image Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE antibody diluted at 1:3000 100 μL Log in to see 1 to 2 Days
Bat Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
Dog Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
Human Rabbit Un-conjugated IHC (p), WB 0.1 mg Log in to see 6 to 8 Days

TOP2B Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
Mouse (Murine)

Rat (Rattus)

Human , , ,

Top referenced anti-Topoisomerase (DNA) II beta 180kDa Antibodies

  1. Human Monoclonal TOP2B Primary Antibody for BI, IF - ABIN968562 : Brown, Holden, Rahn, Perkins: Immunohistochemical staining for DNA topoisomerase IIa in Hodgkin's disease. in American journal of clinical pathology 1998 (PubMed)
    Show all 5 Pubmed References

  2. Human Monoclonal TOP2B Primary Antibody for BI, IF - ABIN968561 : Herzog, Holmes, Tuschong, Ganapathi, Zwelling: Absence of topoisomerase IIbeta in an amsacrine-resistant human leukemia cell line with mutant topoisomerase IIalpha. in Cancer research 1998 (PubMed)
    Show all 5 Pubmed References

  3. Human Polyclonal TOP2B Primary Antibody for ICC, IF - ABIN251526 : Elliott, Crawford, Mulligan, Summerfield, Newton, Wallis, Mainou-Fowler, Evans, Bedwell, Durkacz, Willmore: Mitoxantrone in combination with an inhibitor of DNA-dependent protein kinase: a potential therapy for high risk B-cell chronic lymphocytic leukaemia. in British journal of haematology 2010 (PubMed)
    Show all 3 Pubmed References

  4. Human Polyclonal TOP2B Primary Antibody for IHC (p), IHC - ABIN257919 : Barajon, Serrao, Arnaboldi, Opizzi, Ripamonti, Balsari, Rumio: Toll-like receptors 3, 4, and 7 are expressed in the enteric nervous system and dorsal root ganglia. in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 (PubMed)

  5. Human Polyclonal TOP2B Primary Antibody for ICC, IF - ABIN4361378 : Aviner, Shenoy, Elroy-Stein, Geiger: Uncovering Hidden Layers of Cell Cycle Regulation through Integrative Multi-omic Analysis. in PLoS genetics 2015 (PubMed)

More Antibodies against Topoisomerase (DNA) II beta 180kDa Interaction Partners

Mouse (Murine) Topoisomerase (DNA) II beta 180kDa (TOP2B) interaction partners

  1. We demonstrated that Nup98 (show NUP98 Antibodies)-TopIIbeta and Nup98 (show NUP98 Antibodies)-SETBP1 (show SETBP1 Antibodies) negatively regulate the XPO1 (show XPO1 Antibodies)-mediated protein export. Our results will contribute to the understanding of the molecular mechanism by which the Nup98 (show NUP98 Antibodies)-fusion proteins induce tumorigenesis.

  2. TOP2beta as a factor involved in regulating granulosa cell genomic integrity and follicle atresia.

  3. Results identified topoisomerase IIbeta (Top2beta) as a novel determinant for camptothecin sensitivity.

  4. Top IIbeta might be a down-stream target of Nurr1 (show NR4A2 Antibodies), which might influence the processes of axon genesis in dopaminergic neurons via the regulation of TOP IIbeta expression.

  5. Findings suggest a chromatin-based targeting of Top2beta to regulatory regions in the genome to govern the transcriptional program associated with neuronal differentiation and longevity.

  6. Data suggest that activity of TOP2B in elongating spermatids is strongly inhibited by PARP1 (show PARP1 Antibodies) and PARP2 (show PARP2 Antibodies), and this is in turn counteracted by the PAR (show AFG3L2 Antibodies)-degrading activity of PAR (show AFG3L2 Antibodies) glycohydrolase.

  7. proteasomal degradation of TOP2beta induced by the TOP2-DNA covalent complex or the TOP2 circular clamp is due to transcriptional arrest but not DNA damage

  8. DNA topoisomerase II beta deficiency severely affects cerebral cortex stratification: no subplate is discernible, and neurons born at later stages of corticogenesis fail to migrate to the superficial layers.

  9. A nuclear matrix-associated (show SMARCA5 Antibodies) topoisomerase IIB (TOP2B) interacting with an extracellular Mn2+/Ca2 (show CA2 Antibodies)+-dependent nuclease (show DCLRE1C Antibodies) cleaves sperm DNA into loop-sized fragments.

  10. These results suggest that the defect in establishing neuromuscular junctions in top2beta knockout mice could be due to the lack of TopIIbeta-mediated neurite outgrowth.

Human Topoisomerase (DNA) II beta 180kDa (TOP2B) interaction partners

  1. The article discusses origin of topoisomerase II beta, its structure, activities reported in vitro and in vivo along with implications in cellular processes namely transcription, DNA repair, neuronal development, aging, HIV-infection and cancer. (Review)

  2. recent data point to a critical role of topoisomerase II beta (TOP2B), which is a primary target of anthracycline poisoning, in the pathophysiology of anthracycline-induced congestive heart failure .

  3. The decatenation checkpoint is regulated, not only by topo IIalpha, as previously reported, but also by topo IIbeta. The decatenation checkpoint is most efficient when both isoforms are present. Deletion of most of the C-terminus of topo IIbeta, while preserving the nuclear localization signal, and mutation of Y656 in topo IIbeta inhibits the decatenation checkpoint and sensitivity to topo II (show TOP2 Antibodies)-targeted drugs.

  4. TOP2B is positioned to solve topological problems at diverse cis-regulatory elements and its occupancy is a highly ordered and prevalent feature of CTCF/cohesin binding sites that flank TADs.

  5. Data indicate that cortex involvement, lower World Health Organization grade and DNA topoisomerase II (show TOP2 Antibodies) positivity were strong predictors for preoperative epileptic seizures.

  6. during early development, TOP2A (show TOP2A Antibodies) is likely to have a role in cell proliferation, whereas TOP2B is expressed in post-mitotic cells and may be important in controlling expression of long genes even at this early stage.

  7. Cinobufacini inhibited the proliferation of the HepG-2 cells induced apoptosis in a dose- and time-dependent manner and downregulated the mRNA and protein expression levels of TOPO I (show TOP1 Antibodies) and TOPO II (show TOP2 Antibodies)

  8. A direct interaction between Ku70 (show XRCC6 Antibodies)/86 and BRG1 (show SMARCA4 Antibodies) brings together SWI (show SMARCA1 Antibodies)/SNF (show SNRPA Antibodies) remodeling capabilities and TOP2beta activity to enhance the transcriptional response to hormone stimulation.

  9. Human cytomegalovirus IE1 exon 4 interacts with Topoisomerase IIbeta (TOPOIIbeta), whose activity is required for viral genome persistence and maintenance via binding to a cis (show CISH Antibodies)-acting viral maintenance element.

  10. Proteolytic degradation of Top2 (show TOP2A Antibodies) enables the processing of Top2 (show TOP2A Antibodies).DNA and Top2 (show TOP2A Antibodies).RNA covalent complexes by TDP2 (show TDP2 Antibodies).

Topoisomerase (DNA) II beta 180kDa (TOP2B) Antigen Profile

Protein Summary

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants\; however, the second variant has not yet been fully described.

Gene names and symbols associated with TOP2B

  • DNA topoisomerase II beta (TOP2B) antibody
  • topoisomerase (DNA) II beta (Top2b) antibody
  • DNA topoisomerase II beta (Top2b) antibody
  • microRNA 4442 (MIR4442) antibody
  • D230016L12Rik antibody
  • Top-2 antibody
  • TOP2B antibody
  • top2beta antibody
  • TOPIIB antibody

Protein level used designations for TOP2B

topoisomerase (DNA) II beta 180kDa , DNA topoisomerase II, beta isozyme , DNA topoisomerase 2-beta , topoisomerase II beta , DNA topoisomerase II beta , DNA topoisomerase II, 180 kD , U937 associated antigen , antigen MLAA-44 , topo II beta , topoisomerase IIb , DNA topoisomeraseII_beta , topoisomerase II beta-2

460232 Pan troglodytes
21974 Mus musculus
361100 Rattus norvegicus
7155 Homo sapiens
395966 Gallus gallus
Selected quality suppliers for anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) Antibodies
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