TOP2B antibody (AA 1-50)

Catalog No. ABIN1533446

The Rabbit Polyclonal anti-TOP2B antibody (ABIN1533446) specifically detects TOP2B in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for TOP2B antibody (AA 1-50) (ABIN1533446)

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-TOP2B Antibody

Binding Specificity: AA 1-50

Purpose: Topo IIβ Antibody

Specificity: Topo IIβ Polyclonal Antibody detects endogenous levels of Topo IIβ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TOP2B. AA range:1-50

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Alternative Name: TOP2B

Background:

Synonyms: TOP2B, DNA topoisomerase 2-beta, DNA topoisomerase II, beta isozyme

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla

Gene Name: TOP2B

Protein Name: DNA topoisomerase 2-beta

Molecular Weight: 183 kDa

Gene ID: 7155

OMIM: 126431

UniProt: Q02880