anti-Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) Antibodies

vps35 belongs to a group of vacuolar protein sorting (VPS) genes. Additionally we are shipping vps35 Proteins (3) and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
vps35 65114 Q9EQH3
vps35 55737 Q96QK1
vps35 25479  
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Top anti-vps35 Antibodies at antibodies-online.com

Showing 10 out of 57 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Chinese Hamster Goat Un-conjugated ICC, IF, IHC (p), IHC, ELISA, WB ABIN250175 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ABIN250175 staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3?g/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining. 0.1 mg Log in to see 7 to 9 Days
$516.98
Details
Human Goat Un-conjugated ELISA, IF, WB ABIN185182 staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ABIN185182 staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells.  Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3 ug/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining. 100 μg Log in to see 6 to 7 Days
$429.84
Details
Cow Rabbit Un-conjugated WB Host: Rabbit  Target Name: Vps35  Sample Tissue: Mouse Lung lysates  Antibody Dilution: 1.0 µg/mL 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ICC, IF, IHC (p), WB IHC-P Image VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term , dilution: 1:250. WB Image VPS35 antibody [C3], C-term detects VPS35 protein by western blot analysis. A. 30 μg A549 whole cell extract B. 30 μg H1299 whole cell extract C. 30 μg HCT116 whole cell extract 7.5 % SDS-PAGE VPS35 antibody [C3], C-term , dilution: 1:1000 100 μL Log in to see 3 to 4 Days
$466.18
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Western Blot: VPS35 Antibody  - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II. Western Blot: VPS35 Antibody  - Analysis in human cell line HEK 293. 0.1 mL Log in to see 10 to 13 Days
$494.38
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP1-31069] - Methanol-fixed HCT116, using VPS35 antibody at 1:500 dilution. Western Blot: VPS35 Antibody [NBP1-31069] - Sample(30 ug of whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 D:MOLT4 E:Raji 7. 5% SDS PAGE, antibody diluted at 1:500. 0.1 mL Log in to see 8 to 11 Days
$516.98
Details
Cow Rabbit Un-conjugated WB Western blot analysis of VPS35 expression in Hela (A), HepG2 (B), Raji (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Cow Goat Un-conjugated ELISA, IHC, IHC (p), WB Human Breast, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml. 50 μg Log in to see 11 to 14 Days
$484.00
Details
Human Mouse Un-conjugated ELISA, WB VPS35 monoclonal antibody (M02), clone 2D3. Western Blot analysis of VPS35 expression in human colon. VPS35 monoclonal antibody (M02), clone 2D3. Western Blot analysis of VPS35 expression in HeLa. 100 μg Log in to see 11 to 12 Days
$440.00
Details
Human Rabbit Un-conjugated IHC, IHC (p), IP, WB Immunoprecipitation: VPS35 Antibody  - Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody. Western blot analysis was performed using VPS35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent. 0.1 mL Log in to see 8 to 11 Days
$516.98
Details

Top referenced anti-vps35 Antibodies

  1. Chinese Hamster Polyclonal vps35 Primary Antibody for ICC, IF - ABIN250175 : Hong, Yang, Zhang, Niu, Li, Zhao, Liu: The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport. in Cell research 2009 (PubMed)
    Show all 16 Pubmed References

  2. Human Polyclonal vps35 Primary Antibody for IHC (p), ELISA - ABIN548136 : Zhang, Yu, Gao, Fu, Dai, Zhao, Zheng, Zhao: Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21. in Genomics 2001 (PubMed)
    Show all 4 Pubmed References

  3. Human Monoclonal vps35 Primary Antibody for ELISA, WB - ABIN565910 : Prosser, Tran, Schooley, Wendland, Ngsee: A novel, retromer-independent role for sorting nexins 1 and 2 in RhoG-dependent membrane remodeling. in Traffic (Copenhagen, Denmark) 2010 (PubMed)
    Show all 3 Pubmed References

  4. Human Polyclonal vps35 Primary Antibody for IHC, IHC (p) - ABIN4365839 : Yin, Liu, He, Zhou, Yuan, Zhao: Vps35-dependent recycling of Trem2 regulates microglial function. in Traffic (Copenhagen, Denmark) 2016 (PubMed)

  5. Human Polyclonal vps35 Primary Antibody for ELISA, IF - ABIN185182 : Lee, Uchida, Emoto, Umeda, Kuge, Taguchi, Arai: Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis. in Genes to cells : devoted to molecular & cellular mechanisms 2012 (PubMed)
    Show all 2 Pubmed References

More Antibodies against vps35 Interaction Partners

Fruit Fly (Drosophila melanogaster) Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) interaction partners

  1. The in vivo effects of P316S, D620N and L774M of VPS35 using Drosophila as a model, were investigated.

  2. The findings of this study indicate that the VPS35 may play a crucial role in alpha-synucle in degradation by modulating the maturation of cathepsin D and might thereby contribute to the pathogenesis of the disease.

  3. overexpression of Vps35 ameliorates the pathogenic mutant LRRK2 eye phenotype in a Drosophila model of Parkinson's disease

  4. Vps35 negatively regulates actin polymerisation, and genetic interactions suggest that some of the endocytic and signalling defects of vps35 mutants are due to this function

Mouse (Murine) Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) interaction partners

  1. VPS35[D620N] knock-in mutation strikingly elevates LRRK2-mediated phosphorylation of Rab8A, Rab10, and Rab12 in mouse embryonic fibroblasts.

  2. Parkin gene silencing in primary cortical neurons selectively disrupts the vesicular sorting of the autophagy receptor ATG9A, a WASH-dependent retromer cargo. Parkin is not required for dopaminergic neurodegeneration induced by the expression of PD-linked D620N VPS35 in mice, consistent with VPS35 being located downstream of parkin function

  3. Vps35 D620N allele is a partial-loss-of-function allele and that such a genetic predisposition and age-related alterations in the nigrostriatal dopamine system cooperatively influence the pathogenesis of PARK17.

  4. Results provide evidence for a critical role of Vps35 in mouse corneal dystrophy; suggest that SLC4A11 appears to be a Vps35/retromer cargo, and Vps35-regulation of SLC4A11 trafficking may underlie Vps35/retromer regulation of corneal dystrophy.

  5. Our study suggests that Vps35/retromer is responsible for recycling of Trem2 in the regulation of microglial function such as proinflammatory responses.

  6. Using in vivo knockdown of the critical retromer component VPS35, we demonstrate a specific role for this endosomal sorting complex in the trafficking of AMPA receptors during NMDA-receptor-dependent LTP at mature hippocampal synapses.

  7. the data presented in this manuscript demonstrate a critical function of VPS35 in regulating PTH1R trafficking. This event and VPS35-interaction with PPP1R14C appear to be essential for turning off PTH1R's endosomal signaling, and promoting PTH1R-mediated catabolic response and bone remodeling.

  8. These results provide evidence for VPS35's function in promoting spine maturation, which is likely through increasing AMPA receptor targeting to the postsynaptic membrane.

  9. Deletion of the VPS35 gene in dopamine (DA) neurons resulted in Parkinson disease-like deficits, including loss of DA neurons and accumulation of alpha-synuclein.

  10. These results suggest that VPS35 deficiency or mutation promotes Parkinson Disease (PD) pathogenesis, and reveals a crucial pathway, VPS35-Lamp2a-alpha-synuclein, to prevent PD pathogenesis.

  11. Our studies reveal unexpected genetic and functional interactions between VPS35 and EIF4G1, two seemingly unrelated PD genes, and functionally connect them to alpha-synuclein pathobiology in yeast, worms, and mouse.

  12. Vps35 is necessary for mouse retinal ganglion cell survival and regeneration.

  13. VPS35 critically deregulates RANK signaling, thus restraining increased formation of hyperresorptive OCs and preventing osteoporotic deficits.

  14. VPS35 as an essential role in the suppression of AD neuropathology and in the inhibition of BACE1 activation and Abeta production by promoting BACE1 endosome-to-Golgi retrieval.

  15. These results suggest that mouse Vps26b-Vps29-Vps35 retromer complex is implicated in the transport of sortilin from endosomes to the trans-Golgi network.

  16. A highly conserved region of Vps35p is essential for its interaction with Vps26p and for assembly of the cytoplasmic coat retromer complex.

Human Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) interaction partners

  1. LRRK2-mediated Rab10 phosphorylation is increased in neutrophils as well as monocytes isolated from three Parkinson's patients with a heterozygous VPS35[D620N] mutation.

  2. a novel regulator of N-Ras trafficking and signaling

  3. these results suggest that VPS35 D620N mutation-induced excessive mitochondrial fission leads to the defects in the assembled complex I and supercomplex and causes bioenergetics deficits.

  4. These experimental data establish the retromer complex as a key spatiotemporal regulator of IFNAR endosomal sorting and a new factor in type-I IFN-induced JAK/STAT signalling and gene transcription.

  5. Present data support a role for perturbed VPS35 and retromer function in the pathogenesis of Parkinson's disease.

  6. These findings provide new mechanistic insights into the role of VPS35 in the regulation of AIMP2 levels and cell death.

  7. The retromer complex is a highly conserved membrane trafficking assembly composed of three proteins - Vps26, Vps29 and Vps35, which are impaired in neurodegenerative diseases. (Review)

  8. It could interact with DRD1 and regulate DRD1 cell surface recycling, as well as DRD1- mediated dopamine signaling.

  9. The absence of mutations in VPS35 genes reveals that they are uncommon causes of Parkinson disease in Brazil

  10. VPS35 has been associated with autosomal dominant forms of Parkinson's disease with a high but incomplete penetrance .

  11. results lend further support to the notion that VPS35-DLP1 interaction is key to the retromer-dependent recycling of mitochondrial DLP1 complex during mitochondrial fission and provide a novel therapeutic target to control excessive fission and associated mitochondrial deficits.

  12. Silencing VPS35 increased N-Ras's association with cytoplasmic vesicles, diminished GTP loading of Ras, and inhibited mitogen-activated protein kinase signaling and growth of N-Ras-dependent melanoma cells.

  13. deletion of VPS35 in yeast (vps35Delta) leads to a dose-dependent growth defect towards copper. This increased sensitivity could be rescued by transformation with yeast wild-type VPS35 but not by the expression of a construct harboring the yeast equivalent (i.e. D686N) of the most commonly identified VPS35-associated Parkinson disease mutation, p.D620N

  14. the Vps35 R524W-containing retromer has a decreased endosomal association, which can be partially rescued by R55, a small molecule previously shown to stabilize the retromer complex, supporting the potential for future targeting of the retromer complex in the treatment of Parkinson disease.

  15. X-ray crystallographic analysis of a 4-component complex comprising the VPS26 & VPS35 subunits of retromer, sorting nexin SNX3, & recycling signal from the divalent cation transporter DMT1-II; analysis identifies a binding site for canonical recycling signals at the interface between VPS26 & SNX3; shows cooperative interactions among the VPS subunits, SNX3 & cargo that couple signal-recognition to membrane recruitment.

  16. findings provide evidence that the VPS35 D620N mutation likely confers pathogenicity through a partial loss of function mechanism and that this may be linked to other known pathogenic mechanisms such as mitochondrial dysfunction

  17. VPS35 mutations cause mitochondrial fragmentation and cell death in cultured neurons in vitro, in mouse substantia nigra neurons in vivo and in human fibroblasts from an individual with PD who has the VPS35(D620N) mutation.

  18. neither the R120W nor the N370S variant of the GBA gene nor D620N mutation of the VPS35 gene were detected among the Parkinson disease cases or the controls.

  19. Mutagenesis studies coupled with coimmunoprecipitations revealed that retromer-mediated trafficking requires the Env cytoplasmic tail that we show binds directly to retromer components Vps35 and Vps26.

  20. VPS35 D620N and EIF4G1 R1205H mutations are not a common cause of Parkinson disease in the Greek population.

vps35 Antigen Profile

Protein Summary

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Gene names and symbols associated with vps35

  • VPS35, retromer complex component (VPS35) antibody
  • vacuolar protein sorting-associated protein 35 (Tc00.1047053511367.170) antibody
  • vacuolar protein sorting-associated protein 35 (Tb10.70.5460) antibody
  • vacuolar protein sorting-associated protein 35 (BBOV_IV009740) antibody
  • vacuolar protein sorting-associated protein 35 (CC1G_12185) antibody
  • vacuolar protein sorting-associated protein 35 (PTRG_08682) antibody
  • vacuolar protein sorting-associated protein 35 (CMU_017400) antibody
  • vacuolar protein sorting-associated protein 35 (PHATR_43830) antibody
  • vacuolar protein sorting-associated protein 35 (MCYG_00751) antibody
  • vacuolar protein sorting-associated protein 35 (MGYG_01212) antibody
  • hypothetical protein (PGTG_10018) antibody
  • vacuolar protein sorting-associated protein 35 (Tsp_09825) antibody
  • vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35) antibody
  • VPS35, retromer complex component (vps35) antibody
  • Vacuolar protein sorting 35 (Vps35) antibody
  • VPS35 retromer complex component L homeolog (vps35.L) antibody
  • VPS35 retromer complex component (vps35) antibody
  • vacuolar protein sorting 35 homolog (S. cerevisiae) (vps35) antibody
  • vacuolar protein sorting 35 (EHI_086580) antibody
  • vacuolar protein sorting 35 (EHI_002990) antibody
  • vacuolar protein sorting 35 (EHI_041950) antibody
  • vacuolar protein sorting 35 (EHI_096520) antibody
  • Vacuolar protein sorting 35 (Bm1_44045) antibody
  • vacuolar protein sorting 35 (LOC100285267) antibody
  • VPS35 retromer complex component (Vps35) antibody
  • 23.m05999 antibody
  • AI647796 antibody
  • bZ34G2.3 antibody
  • CG5625 antibody
  • DKFZp459E1510 antibody
  • Dmel\\CG5625 antibody
  • dvps35 antibody
  • MEM3 antibody
  • MGC81959 antibody
  • PARK17 antibody
  • si:rp71-34g2.3 antibody
  • vps35 antibody
  • wu:fa66e03 antibody
  • wu:fb95c08 antibody
  • zgc:136268 antibody

Protein level used designations for vps35

vacuolar protein sorting-associated protein 35 , vacuolar protein sorting 35 homolog (S. cerevisiae) , vacuolar protein sorting 35 homolog , vacuolar protein sorting 35-like , CG5625-PA , CG5625-PB , Vps35-PA , Vps35-PB , vacuolar protein sorting 35 , Vacuolar protein sorting 35 , maternal embryonic message 3 , maternal-embryonic 3 , vesicle protein sorting 35 , hVPS35

GENE ID SPECIES
415750 Gallus gallus
465139 Pan troglodytes
475346 Canis lupus familiaris
717394 Macaca mulatta
3551850 Trypanosoma cruzi strain CL Brener
3663050 Trypanosoma brucei brucei strain 927/4 GUTat10.1
5479130 Babesia bovis T2Bo
6004947 Coprinopsis cinerea okayama7130
6346969 Pyrenophora tritici-repentis Pt-1C-BFP
6995620 Cryptosporidium muris RN66
7203962 Phaeodactylum tricornutum CCAP 1055/1
9223543 Arthroderma otae CBS 113480
10032453 Arthroderma gypseum CBS 118893
10544762 Puccinia graminis f. sp. tritici CRL 75-36-700-3
10907761 Trichinella spiralis
100015669 Monodelphis domestica
100056512 Equus caballus
100174173 Pongo abelii
100351202 Oryctolagus cuniculus
100367783 Saccoglossus kowalevskii
100551222 Meleagris gallopavo
100559729 Anolis carolinensis
100599794 Nomascus leucogenys
100623578 Sus scrofa
37536 Drosophila melanogaster
446307 Xenopus laevis
549980 Xenopus (Silurana) tropicalis
561697 Danio rerio
3405460 Entamoeba histolytica HM-1:IMSS
3407291 Entamoeba histolytica HM-1:IMSS
3410852 Entamoeba histolytica HM-1:IMSS
3411245 Entamoeba histolytica HM-1:IMSS
6103693 Brugia malayi
100285267 Zea mays
65114 Mus musculus
55737 Homo sapiens
25479 Rattus norvegicus
521864 Bos taurus
Selected quality suppliers for anti-vps35 (vps35) Antibodies
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