NIPBL antibody (Nipped-B Homolog (Drosophila)) (AA 344-356)

Details for Product anti-NIPBL Antibody No. ABIN100654
Antigen
Epitope
AA 344-356
6
4
2
2
1
1
1
1
1
1
Reactivity
Human
37
36
19
1
1
1
Host
Goat
24
11
9
2
Clonality
Polyclonal
Conjugate
This NIPBL antibody is un-conjugated
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
23
18
13
13
11
9
8
3
1
Options
Immunogen This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 344-356 of Human IDN3.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Antigen
Alternative Name IDN3 (NIPBL Antibody Abstract)
Background IDN3 (also known as Colon tumor susceptibility 2, DKFZp434L1319, FLJ13648, Mis 4, Scc-2, Delangin and Sister chromatid cohesion protein) is the mammalian homologue of Scc2 in S. cerevisiae and Mis4 in S. pombe. In yeast, this factor was originally identified genetically as being involved in sister chromatid cohesion; subsequent work has determined that it is not involved in cohesion directly but is required for the loading of cohesins onto chromatin during S phase. It shares 37% sequence identity with Nipped-B gene product of Drosophila, which facilitates enhancer-promoter communication of remote enhancers. The Drosophila protein is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair.  IDN3 is a nuclear protein that is widely expressed and found at the highest levels in heart, skeletal muscle, fetal and adult liver, and fetal and adult kidney. The protein is also found, albeit at lower levels of expression in thymus, placenta, peripheral leukocyte and small intestine.  Multiple isoforms of the protein have been reported.
Synonyms: CDLS antibody, Colon tumor susceptibility 2 antibody, Delangin antibody, DKFZp434L1319 antibody, FLJ11203 antibody
Gene ID 25836, 4760549
UniProt Q6KC79
Pathways Sensory Perception of Sound, Stem Cell Maintenance
Application Notes This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect bands at 316 kDa in size corresponding to IDN3 by western blotting in the appropriate cell lysate or extract.  Splice variants for this protein may show molecular weights of 304 kDa and 122 kDa.
Restrictions For Research Use only
Format Liquid
Concentration 1.6 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Nipped-B Homolog (Drosophila) (NIPBL) (AA 344-356) antibody (ABIN100654) Western blot using Affinity Purified anti-IDN3 antibody shows detection of a band ~3...
Image no. 2 for anti-Nipped-B Homolog (Drosophila) (NIPBL) (AA 344-356) antibody (ABIN100654) Western blot using Affinity Purified anti-IDN3 antibody shows detection of bands at ...