Goat anti-Human IgA (Heavy Chain) Antibody (Biotin) - Preadsorbed

Details for Product No. ABIN100717
Antigen
Epitope
Heavy Chain
69
49
23
11
10
6
6
1
1
Reactivity
Human
126
60
38
28
13
11
10
10
10
10
6
5
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
142
88
57
13
7
7
2
Clonality
Polyclonal
Conjugate
Biotin
37
34
31
21
20
15
6
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Anti-Human IgA alpha heavy chain antibody was produced by repeated immunization with Human IgA alpha heavy chain in goat.

Immunogen Type: Native Protein

Isotype IgG
Cross-Reactivity Human
Characteristics Anti-Human IgA alpha heavy chain antibody generated in goat detects specifically Human IgA alpha heavy chain.
Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Labeling Ratio 10-20
Antigen
Background

Synonyms: goat anti-Human IgA (alpha chain) biotin conjugated Antibody, goat anti-Human IgA alpha Antibody biotin conjugation

Background: Anti-Human IgA Biotin Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-HUMAN IgA (alpha chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:60,000 - 1:600,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Aliquot to Avoid repeated freezing and thawing.
Storage RT,4 °C,-20 °C
Expiry Date 12 months
Product cited in: Yu, Pollock, Duval, Lewis, Joseph, Meade, Cavacini: "Neutralization of HIV by milk expressed antibody." in: Journal of acquired immune deficiency syndromes (1999), Vol. 62, Issue 1, pp. 10-6, 2013 (PubMed).