Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN101379

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Armenian Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG (H&L)

Cross-Reactivity: Armenian Hamster

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose

Sterility: Sterile filtered

Immunogen:

Immunogen: Armenian Hamster IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Hamster Secondary Antibody conjugated to alkaline phosphatase is available in a variety of formats. Anti-IgG Secondary Antibody is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,000 - 1:40,000

Western Blot Dilution: 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Armenian Hamster IgG Antibody alkaline phosphatase Conjugation, goat anti-Armenian Hamster IgG alkaline phosphatase Conjugated Antibody, goat Anti-Hamster IgG alk phos Conjugated Secondary Antibody

Background: Anti-Armenian Hamster IgG Alkaline Phosphatase Antibody generated in goat detects Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.