Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN1044077

Supplier Product No.: 720-102-440

Quick Overview for Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed (ABIN1044077)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: FITC

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Golden Syrian Hamster, Armenian Hamster

Host: Goat

Supplier Product No.: 720-102-440

Supplier: Rockland

Purpose: F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against MOUSE and RAT Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Golden Syrian and Armenian Hamster IgG and Golden Syrian and Armenian Hamster Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) and Mouse or Rat Serum Proteins.

Characteristics: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian and Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Golden Syrian and Armenian Hamster IgG whole molecules

Application Details

Application Notes: Application Note: F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Fluorescein Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. FLISA Dilution: 1:10,000-1:50,000 IF Microscopy Dilution: 1:500-1:2500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.