CD36 antibody (FITC)

Catalog No. ABIN1027674

Product Details anti-CD36 Antibody

Target: CD36

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD36 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody CB38 (NL07) recognizes CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
HLDA IV., WS Code P106

Cross-Reactivity (Details): Human

Immunogen: living human myeloid cells

Clone: CB38 (NL07)

Isotype: IgM

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 4 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (0.4 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD36 antibody (ABIN1027674)

Moniuszko, Kowal, Rusak, Pietruczuk, Dabrowska, Bodzenta-Lukaszyk: "Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants." in: Clinical and vaccine immunology : CVI, Vol. 13, Issue 6, pp. 704-7, (2006) (PubMed).

McKenna, Washington, Aquino, Picker, Kroft: "Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry." in: Blood, Vol. 98, Issue 8, pp. 2498-507, (2001) (PubMed).

Kapinsky, Torzewski, Büchler, Duong, Rothe, Schmitz: "Enzymatically degraded LDL preferentially binds to CD14(high) CD16(+) monocytes and induces foam cell formation mediated only in part by the class B scavenger-receptor CD36." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 21, Issue 6, pp. 1004-10, (2001) (PubMed).

Hoffmann, deCathelineau, Ogden, Leverrier, Bratton, Daleke, Ridley, Fadok, Henson: "Phosphatidylserine (PS) induces PS receptor-mediated macropinocytosis and promotes clearance of apoptotic cells." in: The Journal of cell biology, Vol. 155, Issue 4, pp. 649-59, (2001) (PubMed).

Matasić, Dietz, Vuk-Pavlović: "Dexamethasone inhibits dendritic cell maturation by redirecting differentiation of a subset of cells." in: Journal of leukocyte biology, Vol. 66, Issue 6, pp. 909-14, (2000) (PubMed).

Fadok, Warner, Bratton, Henson: "CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6250-7, (1998) (PubMed).

Alessio, Greco, Primo, Ghigo, Bosia, Tandon, Ockenhouse, Jamieson, Malavasi: "Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07." in: Blood, Vol. 82, Issue 12, pp. 3637-47, (1994) (PubMed).

Alessio, Roggero, Bussolino, Saitta, Malavasi: "Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule)." in: Current studies in hematology and blood transfusion, Issue 58, pp. 182-6, (1992) (PubMed).

Alessio, Ghigo, Garbarino, Geuna, Malavasi: "Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07." in: Cellular immunology, Vol. 137, Issue 2, pp. 487-500, (1991) (PubMed).

Target Details for CD36

Target: CD36

Alternative Name: CD36 (CD36 Products)

Synonyms: BDPLT10 antibody, CHDS7 antibody, FAT antibody, GP3B antibody, GP4 antibody, GPIV antibody, PASIV antibody, SCARB3 antibody, Fat antibody, Scarb3 antibody, GPIIIB antibody, PAS-4 antibody, zgc:92513 antibody, CD36 molecule antibody, CD36 molecule (thrombospondin receptor) antibody, CD36 antibody, Cd36 antibody, cd36 antibody

Background: CD36 (fatty acid translocase, FAT) is an 85-113 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.

Pathways: TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins