BID antibody (BH3 Interacting Domain Death Agonist) (AA 1-195)

Details for Product anti-BID Antibody No. ABIN1077861
Antigen
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist S homeolog
  • BID
  • Bid
  • bida
  • bid.S
  • bid
Epitope
AA 1-195
42
33
24
18
15
15
11
9
9
7
7
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
307
161
77
5
3
2
2
Host
Rabbit
311
77
10
5
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
26
23
13
8
7
7
6
6
6
6
6
6
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
320
158
146
76
47
41
31
20
15
13
12
10
3
2
1
1
1
1
1
1
Options
Immunogen Bid (Met1-Asp195)
Isotype IgG
Specificity The antibody is a rabbit polyclonal antibody raised against Bid. It has been selected for its ability to recognize Bid in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Plasmids, Primers & others Plasmids, Primers & others BID products on genomics-online (e.g. as negative or positive controls)
Antigen
Background Alternative Names: p22 BID
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Application Notes
  • Western blotting: 1-5 μg/mL
  • Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
  • Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
  • Immunohistochemistry in paraffin section: 5-20 μg/mL
  • Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
  • Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only
Format Liquid
Concentration 200 μg/mL
Buffer 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN1077861) anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (Image 1)
Image no. 2 for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN1077861) Figure. Western Blot; Sample: Human Lung Tissue.
Image no. 3 for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN1077861) Used in DAB staining on fromalin fixed paraffin-embedded Spleen tissue
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