TRPA1 antibody (N-Term)

Catalog No. ABIN1104836

Product Details anti-TRPA1 Antibody

Target: TRPA1 (Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes human and mouse ANKTM1.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide made to a region within the N-terminus (residues 1-100) of the human TRPA1 protein [Swiss-Prot#O75762].

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 2 μg/mL. Detects a band at ~110kD.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine, 150 mM sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for TRPA1

Target: TRPA1 (Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))

Alternative Name: TRPA1 (TRPA1 Products)

Background: Name/Gene ID: TRPA1
Subfamily: Calcium channel - TRP
Family: Ion Channel

Synonyms: TRPA1, ANKTM1, TRPA-1

Gene ID: 8989

UniProt: O75762