TRPA1 antibody (AA 1050-1120)

Catalog No. ABIN351107

There are 2+ publications for this product available. The Sheep Polyclonal anti-TRPA1 antibody is suitable to detect TRPA1 in samples from Rat. It has been validated for WB and IHC.

Quick Overview for TRPA1 antibody (AA 1050-1120) (ABIN351107)

Target: TRPA1 (Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This TRPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TRPA1 Antibody

Binding Specificity: AA 1050-1120

Purpose: Sheep antibody to TRPA1 (1050-1120)

Specificity: Specific for TRPA1.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 1050-1120 of rat TRPA1 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

References for anti-TRPA1 antibody (ABIN351107)

Cho, Lee, Choi, Choi, Lim, Kim, Kim, Jung, Hwang: "GPR171 Activation Modulates Nociceptor Functions, Alleviating Pathologic Pain." in: Biomedicines, Vol. 9, Issue 3, (2021) (PubMed).

Nagy, Lynn, Senecal, Stecina: "Connexin36 Expression in Primary Afferent Neurons in Relation to the Axon Reflex and Modality Coding of Somatic Sensation." in: Neuroscience, Vol. 383, pp. 216-234, (2018) (PubMed).

Target Details for TRPA1

Target: TRPA1 (Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))

Alternative Name: TRPA1

Background: The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined, however studies indicate the function may involve a role in signal transduction and growth control. TISSUE SPECIFICITY: Expressed in inner ear. In inner ear it is found in the distal half of stereocilia along the length of the kinocilium and at high level in the pericuticular zone (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion. DEVELOPMENTAL STAGE: During utricle development it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19 possibly corresponding to a second wave of hair cells that are generated at E15. Inhibited by ruthenium red a potent blocker of TRPV channels.

UniProt: Q6RI86