HSPG2 antibody

Catalog No. ABIN114947

The Rat Monoclonal anti-HSPG2 antibody has been validated for WB, IP, IF, IHC (p) and IHC (fro). It is suitable to detect HSPG2 in samples from Human, Mouse, Cow, Rat, Bacteria, Cod and Wolf.

Quick Overview for HSPG2 antibody (ABIN114947)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivity: Human, Mouse, Cow, Rat, Bacteria, Cod, Wolf

Host: Rat

Clonality: Monoclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A7L6

Product Details anti-HSPG2 Antibody

Specificity: A7L6 recognizes domain IV of the core protein of the large Heparan Sulphate Proteoglycan or Perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to Heparitinase treatment.

Purification: Purified

Immunogen: High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation. Immunoblotting: 1/100-1/1000. Immunocytochemistry. Immunohistochemistry on frozen and paraffin-embedded tissues: 1/25-1/200 (withavidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Storage: 4 °C/-20 °C

Storage Comment: Store the original vial at 2-8 °C. After reconstitution store (in aliquots) at -20 °C.

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: HSPG2 / Perlecan

Background: Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.Synonyms: Basement membrane-specific heparan sulfate proteoglycan core protein, PLC

Gene ID: 3339

NCBI Accession: NP_005520

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism