HSPG2 antibody (AA 524-701)

Catalog No. ABIN3042466

This Rabbit Polyclonal antibody specifically detects HSPG2 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for HSPG2 antibody (AA 524-701) (ABIN3042466)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HSPG2 Antibody

Binding Specificity: AA 524-701

Purpose: Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2.

Characteristics: Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody. Tested in IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HSPG2 recombinant protein (Position: F524-K701). Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, HumanWestern blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: HSPG2

Background:

Background: Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

Gene Full Name: heparan sulfate proteoglycan 2

Sequence Similarities: Contains 4 EGF-like domains.

Molecular Weight: 469 kDa

Gene ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism