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MSH6 antibody (MutS Homolog 6 (E. Coli)) Primary Antibody

MSH6 Reactivity: Human, Mouse, Rat, Dog IHC (fro), IHC (p), IP, WB Host: Mouse Monoclonal 44 unconjugated
Catalog No. ABIN115272
$671.00
Plus shipping costs $45.00
0.5 mL
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Available
  • Target
    MSH6
    Reactivity
    • 71
    • 33
    • 25
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog
    Host
    • 54
    • 16
    • 1
    Mouse
    Clonality
    • 45
    • 26
    Monoclonal
    Conjugate
    • 40
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This MSH6 antibody is un-conjugated
    Application
    • 39
    • 25
    • 22
    • 20
    • 16
    • 14
    • 13
    • 8
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    MSH6 is a heterodimer of MSH2 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop.
    Cellular Localization: Nuclear.
    Purification
    Affinity Chromatography.
    Immunogen
    Synthetic human MSH6 peptide
    Clone
    44
    Isotype
    IgG1
  • Application Notes
    Immunoprecipitation. Western Blot: 1/100-1/500. Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod (30 to 60 minutes at room temperature (requires high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining). Immunohistochemistry on Frozen Sections. Recommended Positive Control: Tonsil, colon carcinoma
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    MSH6
    Alternative Name
    MSH6 (MSH6 Antibody Abstract)
    Synonyms
    GTBP, GTMBP, HNPCC5, HSAP, p160, cb326, wu:fb36c12, LOC100227810, AU044881, AW550279, Gtmbp, mutS homolog 6, mutS homolog 6 (E. coli), DNA mismatch repair protein, MSH6, msh6, Msh6
    Background
    An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time.Synonyms: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, MutS-alpha 160 kDa subunit, p160
    Gene ID
    2956
    NCBI Accession
    NP_000170
    UniProt
    P52701
    Pathways
    DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response
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