Ccl21a antibody

Catalog No. ABIN115922

The Rabbit Polyclonal anti-Ccl21a antibody is suitable to detect Ccl21a in samples from Mouse. It has been validated for WB, EIA and Func.

Quick Overview for Ccl21a antibody (ABIN115922)

Target: Ccl21a (CCL21A) (Chemokine (C-C Motif) Ligand 21A (Serine) (CCL21A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ccl21a antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-Ccl21a Antibody

Specificity: This antibody recognises Murine Exodus-2.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant Mouse Exodus-2.

Application Details

Application Notes: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of mExodus-2 (100.0 ng/mL), a concentration of 9.0-10.0 μg/mL of this antibody is required. ELISA: To detect mExodus-2 by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mExodus-2. Western blot: To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Ccl21a

Target: Ccl21a (CCL21A) (Chemokine (C-C Motif) Ligand 21A (Serine) (CCL21A))

Alternative Name: 6Ckine / CCL21a

Background: Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).Synonyms: Beta chemokine exodus-2, C-C motif chemokine 21a, Scya21a, Small-inducible cytokine A21a, TCA-4, TCA4, Thymus-derived chemotactic agent 4

Gene ID: 100039131

NCBI Accession: NP_035465

UniProt: P84443