Chemokine (C-C Motif) Ligand 21A (Serine) (CCL21A) antibody

Details for Product No. ABIN115922
Mouse (Murine)
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
Immunogen Highly pure (>98%) recombinant Mouse Exodus-2.
Specificity This antibody recognises Murine Exodus-2.
Purification Affinity chromatography.
Alternative Name 6Ckine / CCL21a (CCL21A Antibody Abstract)
Background Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).Synonyms: Beta chemokine exodus-2, C-C motif chemokine 21a, Scya21a, Small-inducible cytokine A21a, TCA-4, TCA4, Thymus-derived chemotactic agent 4
Gene ID 100039131
NCBI Accession NP_035465
UniProt P84443
Application Notes Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of mExodus-2 (100.0 ng/mL), a concentration of 9.0-10.0 μg/mL of this antibody is required. ELISA: To detect mExodus-2 by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mExodus-2. Western blot: To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.