CREB1 antibody (N-Term)

Catalog No. ABIN116948

Product Details anti-CREB1 Antibody

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Specificity: This product was prepared from monospecific antiserum by delipidation and Immunoadsorption against an E.coli lysate immobilized on agarose beads. This antibody may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.

Immunogen: CREB-1 (p43) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Western Blot: This antibody was assayed against a lysate of 1 x 10^6 Raji B cells byImmunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at adilution of 1/500-1/1,000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG(H&L) [Goat] (Cat#R1364HRP)ELISA: 1/5,000-1/25,000. Immunoprecipitation. Gelshift: This antibody was tested in Gel Supershift Assay and found to be reactive againstCREB-1 (p43) using 0.5-1.0 μL per assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.56 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1 (CREB1 Products)

Background: Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1

Gene ID: 1385

NCBI Accession: NP_004370

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation