STAT4 antibody (Signal Transducer and Activator of Transcription 4) (pTyr693) (PE)

Details for Product anti-STAT4 Antibody No. ABIN1177202
Target
Binding Specificity
pTyr693
39
20
10
8
7
7
6
5
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse
149
71
60
9
8
8
6
5
4
2
1
1
Host
Mouse
127
18
8
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This STAT4 antibody is conjugated to PE
9
7
7
5
5
4
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
113
67
62
33
31
22
13
8
4
2
2
1
1
1
1
1
Options
Brand BD Phosflow™
Immunogen Phosphorylated Human Stat4 (pY693)
Clone 38-p
Isotype IgG2b, kappa
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Target
Alternative Name Stat4 (STAT4 Antibody Abstract)
Background The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6. Stat4 has been shown to play an important role in development of T helper cells, specifically the Th1 subset. Stat4 is activated by IL-12 and by type 1 interferons. Knockout mice supported the role that Stat4 plays in IL-12 signaling because lymphocytes from Stat 4-/- mice could neither differentiate into Th1 cells nor produce IFN-gamma in response to treatment with IL-12. IFN-gamma plays an important role in host defense. A key component in the activation of Stat4 is the phosphorylation on tyrosine and serine residues in response to IL-12 stimulation. IL-12 stimulation leads to the phosphorylation of Stat4 on tyrosine 693 and serine 721. Transcriptional activity of Stat4 has been shown to be significantly reduced when residues Y693 and S721 are mutated.
Synonyms: Signal transducer and activator of transcription 4, SLEB11
Pathways JAK-STAT Signaling
Sample Volume 5 μL
Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product cited in: Kisseleva, Bhattacharya, Braunstein, Schindler: "Signaling through the JAK/STAT pathway, recent advances and future challenges." in: Gene, Vol. 285, Issue 1-2, pp. 1-24, 2002 (PubMed).

Visconti, Gadina, Chiariello, Chen, Stancato, Gutkind, OShea: "Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity." in: Blood, Vol. 96, Issue 5, pp. 1844-52, 2000 (PubMed).