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ITGA4 antibody (Integrin alpha 4) Primary Antibody

ITGA4 Reactivity: Human, Rat, Cow, Monkey, Pig FACS, IHC (fro), IP Host: Mouse Monoclonal HP2-1 unconjugated
Pubmed (1)
Catalog No. ABIN120127
$489.50
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Available
  • Target
    ITGA4
    Reactivity
    • 156
    • 130
    • 44
    • 19
    • 11
    • 9
    • 9
    • 8
    • 8
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Cow, Monkey, Pig
    Host
    • 80
    • 76
    • 67
    • 1
    • 1
    Mouse
    Clonality
    • 153
    • 72
    • 1
    Monoclonal
    Conjugate
    • 103
    • 25
    • 24
    • 18
    • 8
    • 8
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ITGA4 antibody is un-conjugated
    Application
    • 158
    • 52
    • 49
    • 47
    • 39
    • 25
    • 23
    • 13
    • 12
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
    Specificity
    This antibody recognises the 80 kDa alpha chain of VLA-4. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells. Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.
    Purification
    Affinity chromatography on Protein G
    Immunogen
    JM leukaemia line. Spleen cells from immunised BALB/c mice were fused with cells of the X63 Ag8.653 myeloma cell line.
    Clone
    HP2-1
    Isotype
    IgG1
  • Application Notes
    Flow cytometry: 1 μg/5 x 10e cells. Immunohistochemsitry on frozen sections. Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS containing 0.09 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Kawaguchi, Noriyuki, Kuroda, Kuranishi, Nakahara, Fukuda, Ishizaki, Hotta, Akimoto, Mori: "[Right lung cancer with right aortic arch]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 113-7, 2008 (PubMed).

  • Target
    ITGA4
    Alternative Name
    CD49d / ITGA4 (ITGA4 Antibody Abstract)
    Synonyms
    CD49D, IA4, LOC100219853, integrin subunit alpha 4, integrin alpha 4, integrin subunit alpha 4 S homeolog, ITGA4, Itga4, itga4.S
    Background
    ITGA4 encodes integrin alpha chain 4. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Although alpha 4 is variably cleaved to yield two unlinked chains, this cleavage is unnecessary for integrin assembly and function. Alpha 4 combines with beta 1 (ITGB1) on T-cells to form the integrin very late (activation) antigen 4 (VLA-4) that can bind to the extracellular matrix molecules fibronectin or thrombospondin, and is also a ligand for the cell surface molecule vascular cell adhesion molecule 1 (VCAM-1). In addition, alpha 4 combines with beta 7 to form the lymphocyte homing receptor known as LPAM-1 (lymphocyte Peyer Patch adhesion molecule 1). Integrins are also known to participate in cell-surface mediated signalling.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
    Gene ID
    3676
    NCBI Accession
    NP_000876
    UniProt
    P13612
    Pathways
    Integrin Complex
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