ITGA4 antibody

Catalog No. ABIN120127

Product Details anti-ITGA4 Antibody

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Rat, Cow, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the 80 kDa alpha chain of VLA-4. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells. Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.

Purification: Affinity chromatography on Protein G

Immunogen: JM leukaemia line. Spleen cells from immunised BALB/c mice were fused with cells of the X63 Ag8.653 myeloma cell line.

Clone: HP2-1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1 μg/5 x 10e cells. Immunohistochemsitry on frozen sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ITGA4 antibody (ABIN120127)

Kawaguchi, Noriyuki, Kuroda, Kuranishi, Nakahara, Fukuda, Ishizaki, Hotta, Akimoto, Mori: "[Right lung cancer with right aortic arch]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 113-7, (2008) (PubMed).

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d / ITGA4 (ITGA4 Products)

Synonyms: CD49D antibody, IA4 antibody, LOC100219853 antibody, integrin subunit alpha 4 antibody, integrin alpha 4 antibody, integrin subunit alpha 4 S homeolog antibody, ITGA4 antibody, Itga4 antibody, itga4.S antibody

Background: ITGA4 encodes integrin alpha chain 4. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Although alpha 4 is variably cleaved to yield two unlinked chains, this cleavage is unnecessary for integrin assembly and function. Alpha 4 combines with beta 1 (ITGB1) on T-cells to form the integrin very late (activation) antigen 4 (VLA-4) that can bind to the extracellular matrix molecules fibronectin or thrombospondin, and is also a ligand for the cell surface molecule vascular cell adhesion molecule 1 (VCAM-1). In addition, alpha 4 combines with beta 7 to form the lymphocyte homing receptor known as LPAM-1 (lymphocyte Peyer Patch adhesion molecule 1). Integrins are also known to participate in cell-surface mediated signalling.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gene ID: 3676

NCBI Accession: NP_000876

UniProt: P13612

Pathways: Integrin Complex