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EGFR antibody (pSer1071) (Biotin)

EGFR Reactivity: Human, Mouse, Dog WB, IF, IP, EIA Host: Mouse Monoclonal 1H9 Biotin

Catalog No. ABIN126766

Datasheet as PDF

Target See all EGFR Antibodies

EGFR (Epidermal Growth Factor Receptor (EGFR))
Binding Specificity
39

38

31

31

30

27

27

26

24

22

19

18

16

16

16

15

15

15

11

11

10

9

9

9

8

7

7

6

5

5

5

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3
pSer1071
Reactivity
747

296

282

25

25

24

18

14

8

3

2

2

1

1

1

1

1

1
Human, Mouse, Dog
Host
580

166

14

13

5

2

1

1
Mouse
Compatible Secondaries
Clonality
542

235

2
Monoclonal
Conjugate
410

57

29

22

19

17

16

16

16

16

16

11

10

10

10

10

10

10

10

10

10

8

8

5

4

4

4

4

4

1

1

1

1

1

1
This EGFR antibody is conjugated to Biotin
Application
534

248

149

145

125

102

97

93

82

46

37

34

33

18

11

8

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity

This antibody specifically recognizes EGFR phosphorylated at Serine 1071.

Characteristics

Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification

Size exclusion chromatography

Components

incl. positive Control

Immunogen

Phosphopeptide conjugated to KLH.

Clone

1H9

Isotype

IgG1

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anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody
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EGFR Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
EGFR Reactivity: Human, Rat WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated

Application Notes

Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunoprecipitation: 1 - 10 g per 106 pervanadate-treated A431 cells. Immunocytochemistry: 0.1-1 μg/mL. Luminex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.5 mg/mL

Buffer

PBS/0.09 % Na-Azide/PEG and Sucrose

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-80 °C

Storage Comment

Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date

12 months
Target

EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name

EGFR / ERBB1 (EGFR Products)

Background

EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gene ID

1956

UniProt

P00533

Pathways

NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins