gamma 1 Adaptin antibody (AA 625-675)

Catalog No. ABIN129554

Supplier Product No.: 600-101-289

Product Details anti-gamma 1 Adaptin Antibody

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Binding Specificity: AA 625-675

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This gamma 1 Adaptin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-101-289

Supplier: Rockland

Purpose: AP1G1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human AP1G1.

Characteristics: Synonyms: goat anti-AP1G1 Antibody, AP-1 complex subunit gamma-1, Adapter-related protein complex 1 subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Golgi adaptor HA1/AP1 adaptin subunit gamma-1, Gamma1-adaptin, Clathrin assembly protein complex 1 gamma-1 large chain, ADTG, CLAPG1, AP1G

Purification: The product was affinity purified from antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 625-675 of Human AP1G1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to AP1G1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:8,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.7 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for gamma 1 Adaptin

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Alternative Name: AP1G1 (AP1G1 Products)

Background: Background: AP1G1 (also known as gamma 1 subunit of adaptor -related protein complex 1, Gamma-adaptin, Adaptor protein complex AP-1 gamma-1 subunit, Golgi adaptor HA1/AP1 adaptin gamma-1 subunit and Clathrin assembly protein complex 1 gamma-1 large chain) is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.  The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins.  Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles.  AP1G1 is localized to the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex and is widely expressed.

Gene ID: 164, 71772942

UniProt: O43747