RFP antibody (Red Fluorescent Protein) (AA 234)

Details for Product anti-RFP Antibody No. ABIN129578, Supplier: Log in to see
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Epitope
AA 234
9
4
3
2
Reactivity
Discosoma
92
9
5
1
1
1
Host
Rabbit
47
40
14
6
1
1
Clonality
Polyclonal
Conjugate
This RFP antibody is un-conjugated
5
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
82
44
29
23
18
16
6
4
4
3
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
'Independent Validation' Badge
Antigen RFP
Lot Number 34945
Method validated Immunofluorescence
Positive Control CX3CL1mcherry+ murine thymus
Negative Control CX3CL1 WT murine thymus
Notes

Passed. The RFP antibody ABIN1043867 specifically labels RFP+ cells in CX3CL1mcherry+ murine thymus in immunofluorescence, consistent with the expression pattern of CX3CL1mcherry.

Primary Antibody ABIN1043867
Secondary Antibody donkey Fab'2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)
Protocol
  • Harvest thymus from 7 weeks old mice in 1X Dulbecco's phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
  • Fix mouse thymus in antigen fix (Diapath, P0014) for 2h at 4?C.
  • Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4?C.
  • Dehydrate tissue in 30% sucrose solution at 4?C ON.
  • Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80?C.
  • Cut blocks into 25?m sections using a cryostat (Leica, CM3050 S).
  • Transfer sections to a slide.
  • Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
  • Place slide in a humidified chamber and rehydrate sections in 0.1 M TrisHCl pH7.4 for 10min at RT.
  • Gently remove buffer by tapping slide.
  • Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, lot 015K0039) and 0.5% BSA, for 20min at RT.
  • Incubate sections with primary RFP antibody (Red Fluorescent Protein) (AA 234) (antibodies-online, ABIN4368323, lot 1602) diluted 1:1000 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 2h at RT.
  • Incubate a no primary antibody negative control in parallel in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA.
  • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
  • Incubate sections with secondary donkey Fab'2 anti-rabbit AF647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:300 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 2h at RT.
  • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
  • Add approximately 10?L of Slowfade Gold antifade reagent (ThermoFisher Scientific, S36937, lot 1226836) for each section and mount cover slip.
  • Image acquisition on an LSM 880 (Zeiss), 20x magnification, 1000 resolution.
Validation Images
Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN1043867) CX3CL1 positive cells in CX3CL1mcherry murine thymus (A, mCherry) were stained with A...
Immunogen The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: Recombinant Protein
Isotype IgG
Specificity This product was prepared from monospecific antiserum by imunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Characteristics Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Sterility Sterile filtered
Plasmids, Primers & others Plasmids, Primers & others RFP products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name RFP (RFP Antibody Abstract)
Background Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Application Notes Suggested dilutions:
ELISA : 1:90000
IF Microscopy : 1:200-1:2000
Western Blot : 1:2000 - 1:10000
Immunochemistry: 1:200-1:2000
Other Dilution: User Optimized
Comment

Gene Name: DsRed

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN129578) Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. L...
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN129578) Immunofluorescence Microscopy of Rabbit Anti-RFP antibody. Tissue: HopERCre/+; R26Tom...
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN129578) Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. ...
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN129578) Immunofluorescence Microscopy of Rabbit Anti-RFP antibody. Tissue: (10X) Mouse lung t...
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN129578) Immunofluorescence Microscopy of Rabbit Anti-RFP antibody. Tissue: (10X) Mouse E14.5 ...
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN129578) Western Blot of Rabbit anti-RFP antibody. Marker: Opal Pre-stained ladder . Lane 1: H...
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN129578) Immunofluorescence Microscopy of Rabbit Anti-RFP antibody. Tissue: DsRed transgenic m...
Immunohistochemistry (IHC) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN129578) Immunohistochemistry of Anti-RFP Antibody. Tissue: Mouse gut tissue in tomato transge...
Product cited in: Dhandapani, Arokiaraj, Taberner, Pacifico, Raja, Nocchi, Portulano, Franciosa, Maffei, Hussain, de Castro Reis, Reymond, Perlas, Garcovich, Barth, Johnsson, Lechner, Heppenstall: "Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons." in: Nature communications, Vol. 9, Issue 1, pp. 1640, 2019 (PubMed).

Ollila, Domènech-Moreno, Laajanen, Wong, Tripathi, Pentinmikko, Gao, Yan, Niemelä, Wang, Viollet, Leone, Katajisto, Vaahtomeri, Mäkelä: "Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway." in: The Journal of clinical investigation, Vol. 128, Issue 1, pp. 402-414, 2019 (PubMed).

Cao, Wang, Wang, Han, Zeng: "Three-dimensional volume fluorescence-imaging of vascular plasticity in adipose tissues." in: Molecular metabolism, Vol. 14, pp. 71-81, 2019 (PubMed).

Joe, Flores, Doche, Cline, Clutter, Vander, Riedel, Argetsinger, Carter-Su: "Phosphorylation of the Unique C-Terminal Tail of the Alpha Isoform of the Scaffold Protein SH2B1 Controls the Ability of SH2B1α To Enhance Nerve Growth Factor Function." in: Molecular and cellular biology, Vol. 38, Issue 6, 2019 (PubMed).

Moehlman, Casey, Servage, Orth, Krämer: "Adaptation to constant light requires Fic-mediated AMPylation of BiP to protect against reversible photoreceptor degeneration." in: eLife, Vol. 7, 2019 (PubMed).

Jaeger, Stanley, Weiss, Musso, Chan, Zhang, Feldman-Kiss, Gordon: "A complex peripheral code for salt taste in Drosophila." in: eLife, Vol. 7, 2019 (PubMed).

Maskari, Hardege, Cleary, Figg, Li, Siew, Khir, Yu, Liu, Wilkinson, OShaughnessy, Yasmin: "Functional characterization of common BCL11B gene desert variants suggests a lymphocyte-mediated association of BCL11B with aortic stiffness." in: European journal of human genetics : EJHG, Vol. 26, Issue 11, pp. 1648-1657, 2019 (PubMed).

Murai, Yamaguchi, Shirasaki, Yamagishi, Shindo, Hildebrand, Miura, Nakabayashi, Totsuka, Tomida, Adachi-Akahane, Uemura, Silke, Yagita, Miura, Nakano: "A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs." in: Nature communications, Vol. 9, Issue 1, pp. 4457, 2019 (PubMed).

Cerrato, Parmigiani, Figueres-Oñate, Betizeau, Aprato, Nanavaty, Berchialla, Luzzati, deSperati, López-Mascaraque, Buffo: "Multiple origins and modularity in the spatiotemporal emergence of cerebellar astrocyte heterogeneity." in: PLoS biology, Vol. 16, Issue 9, pp. e2005513, 2019 (PubMed).

Ahmed, Nishida-Fukuda, Li, McDonald, Gradinaru, Macara: "Exocyst dynamics during vesicle tethering and fusion." in: Nature communications, Vol. 9, Issue 1, pp. 5140, 2019 (PubMed).

Lin, Schöneberg, Davies, Ren, Hurley: "The dynamic Atg13-free conformation of the Atg1 EAT domain is required for phagophore expansion." in: Molecular biology of the cell, Vol. 29, Issue 10, pp. 1228-1237, 2019 (PubMed).

Kretzschmar, Post, Bannier-Hélaouët, Mattiotti, Drost, Basak, Li, van den Born, Gunst, Versteeg, Kooijman, van der Elst, van Es, van Rooij, van den Hoff, Clevers: "Profiling proliferative cells and their progeny in damaged murine hearts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 52, pp. E12245-E12254, 2019 (PubMed).

Ojala, Sun, Santiago-Ortiz, Shapiro, Romero, Schaffer: "In Vivo Selection of a Computationally Designed SCHEMA AAV Library Yields a Novel Variant for Infection of Adult Neural Stem Cells in the SVZ." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 26, Issue 1, pp. 304-319, 2019 (PubMed).

Reynolds, Zheng, Rusakov: "Multiplexed calcium imaging of single-synapse activity and astroglial responses in the intact brain." in: Neuroscience letters, Vol. 689, pp. 26-32, 2019 (PubMed).

Tang, Yang, Zhang, Li, Zhang, Wang, Xu, Chen, Teng, Yang: "E-cadherin is Required for the Homeostasis of Lgr5+ Gastric Antral Stem Cells." in: International journal of biological sciences, Vol. 15, Issue 1, pp. 34-43, 2019 (PubMed).

Murakami, Naganuma, Tanigawa, Fujimori, Eto, Nishinakamura: "Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation." in: Scientific reports, Vol. 9, Issue 1, pp. 1172, 2019 (PubMed).

Hannafon, Gin, Xu, Bruns, Calloway, Ding: "Metastasis-associated protein 1 (MTA1) is transferred by exosomes and contributes to the regulation of hypoxia and estrogen signaling in breast cancer cells." in: Cell communication and signaling : CCS, Vol. 17, Issue 1, pp. 13, 2019 (PubMed).

Zhou, Liu, Sun, Cao, Yang: "Transcriptional mechanism of IRF8 and PU.1 governs microglial activation in neurodegenerative condition." in: Protein & cell, Vol. 10, Issue 2, pp. 87-103, 2019 (PubMed).

Webster, Barnett, Stanchfield, Paris, Webster, Cooley-Themm, Levine, Otteson, Linn: "Stimulation of Retinal Pigment Epithelium With an α7 nAChR Agonist Leads to Müller Glia Dependent Neurogenesis in the Adult Mammalian Retina." in: Investigative ophthalmology & visual science, Vol. 60, Issue 2, pp. 570-579, 2019 (PubMed).

Magloire, Cornford, Lieb, Kullmann, Pavlov: "KCC2 overexpression prevents the paradoxical seizure-promoting action of somatic inhibition." in: Nature communications, Vol. 10, Issue 1, pp. 1225, 2019 (PubMed).

Did you look for something else?