ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila)) (AA 1632-1644)

Details for Product anti-ROBO1 Antibody No. ABIN129621
Antigen
Epitope
AA 1632-1644
9
6
5
4
2
2
2
2
1
1
1
1
Reactivity
Human
59
24
24
4
1
Host
Rabbit
45
10
9
Clonality
Polyclonal
Conjugate
This ROBO1 antibody is un-conjugated
3
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
53
37
9
3
2
2
1
1
1
Options
Immunogen This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1.
Isotype IgG
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Alternative Name ROBO-1 (ROBO1 Antibody Abstract)
Background ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2.  The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex.  ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein.  ROBO-1 is a widely expressed protein with the exception of the kidney.  Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.
Synonyms: ROBO-1, Roundabout homolog 1 precursor, Deleted in U twenty twenty (DUTT)
Gene ID 6091
UniProt Q2M1J3
Pathways Positive Regulation of Endopeptidase Activity
Application Notes This affinity purified antibody has been tested for use in ELISA , IP, immunohistochemistry, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~181 kDa in size corresponding to ROBO-1 by western blotting in the appropriate cell lysate or extract.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (AA 1632-1644) antibody (ABIN129621) Affinity Purified anti-ROBO1 antibody was used at a concentration of 5 µg/ml to detec...
Image no. 2 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (AA 1632-1644) antibody (ABIN129621) Western Blot of Rabbit anti-Robo-1 antibody. Lane 1: HeLa WCL. Load: 30 µg per lane. ...
Image no. 3 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (AA 1632-1644) antibody (ABIN129621) Western blot using Affinity Purified anti-ROBO-1 antibody shows detection of a band ...
Image no. 4 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (AA 1632-1644) antibody (ABIN129621) ELISA results of purified Rabbit anti-Robo-1 Antibody tested against BSA-conjugated p...
Image no. 5 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (AA 1632-1644) antibody (ABIN129621) Western Blot of Rabbit anti-ROBO1 antibody. Marker: Opal Pre-stained ladder . Lane 1:...