EGFR antibody (AA 1175-1200)

Catalog No. ABIN129684

Supplier Product No.: 600-401-928

Product Details anti-EGFR Antibody

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Binding Specificity: AA 1175-1200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-928

Supplier: Rockland

Purpose: EGFR phospho Y1197 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against the phosphorylated form of human EGFR protein at the pY1197 residue.

Characteristics: Synonyms: rabbit anti-EGFR pY1197 antibody, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, erbb 1, ERBB-1, HER1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus near amino acids 1175-1200 of human EGFR protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kDa in size corresponding to phosphorylated EGFR protein by western blotting in the appropriate cell lysate or extract.   Less than 5.0 % reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pY1197 of EGFR protein.

Western Blot Dilution: 1:250 - 1:1,500

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR (EGFR Products)

Background: Background: EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development.  Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors.  For example, the epidermal growth factor receptor (EGFR) is mutated and/or overexpressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis.  EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2, ErbB-3/HER-3 and ErbB-4/HER-4. EGFR can heterodimerize with each of the members of this family.

Gene ID: 1956, 29725609

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins