UBE2C antibody (AA 101-179) (Cy5.5)

Catalog No. ABIN1416481

Product Details anti-UBE2C Antibody (Cy5.5)

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Binding Specificity: AA 101-179

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2C antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2C

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBE2C

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Alternative Name: UBE2C (UBE2C Products)

Background:

Synonyms: Cyclin selective ubiquitin carrier protein, dJ447F3.2, Mitotic specic ubiquitin conjugating enzyme, UB E2C, UBCH 10, UBCH10, UBE 2C, Ube2c, UBE2C_HUMAN, Ubiquitin carrier protein C, Ubiquitin carrier protein E2 C, Ubiquitin carrier protein E2C, Ubiquitin conjugating enzyme E2 C, Ubiquitin conjugating enzyme E2C, Ubiquitin protein ligase C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C.

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.

Gene ID: 11065

Pathways: Ubiquitin Proteasome Pathway