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Caspase 3 antibody (Cleaved-Asp175)

Name: Caspase 3 antibody (Cleaved-Asp175)
SKU: ABIN1536103
Price: 341 USD
Availability: InStock

CASP3 Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts from COLO cells, using Caspase 3 (p17,Cleaved-Asp175) Antibody. (Caspase 3 antibody (Cleaved-Asp175))

1 image

Catalog No. ABIN1536103

Datasheet as PDF

Target See all Caspase 3 (CASP3) Antibodies

Caspase 3 (CASP3)
Binding Specificity
21

16

16

15

12

12

10

9

8

8

8

7

6

6

5

5

5

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 126-175, Cleaved-Asp175
Reactivity
199

114

71

15

9

8

8

6

5

4

3

3

2

2

2

2

1
Human, Mouse, Rat
Host
208

24

4

2

2
Rabbit
Compatible Secondaries
Clonality
204

35
Polyclonal
Conjugate
144

18

17

8

7

5

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This Caspase 3 antibody is un-conjugated
Application
175

94

93

58

40

32

28

27

27

26

25

10

10

6

3

2

1

1

1

1
Western Blotting (WB), ELISA

Specificity

Caspase 3 (p17,Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity

> 95 %

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 3.

Isotype

IgG

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Application Notes

WB: 1:500~1:1000 ELISA: 1:20000

Comment

Unigene-Number: Hs.141125 (NCBI Gene Symbol: CASP3)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Stable at -20°C for at least 1 year.

Expiry Date

12 months
Target

Caspase 3 (CASP3)

Alternative Name

Caspase 3 (CASP3 Products)

Background

Synonyms: Apopain precursor, CASP-3, CPP-32, CPP32, Caspase-3, Cysteine protease CPP32, ICE3, SCA-1, SREBP cleavage activity 1, Yama protein
NCBI Gene Symbol: CASP3

Molecular Weight

17 kDa

Gene ID

836

OMIM

600636

UniProt

P42574

Pathways

Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation