YWHAZ antibody (Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide) (pSer58)

Details for Product anti-YWHAZ Antibody No. ABIN1574324
Target
Binding Specificity
pSer58
27
11
10
10
10
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Rat
146
107
85
21
19
16
16
11
7
6
6
6
4
3
2
2
1
1
Host
Rabbit
138
11
Clonality
Polyclonal
Conjugate
This YWHAZ antibody is un-conjugated
9
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
124
75
63
38
34
16
14
13
7
5
3
3
3
Options
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R).
Specificity Epitopes for Species: H:S58,M:S58,R:S58
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Target
Alternative Name 14-3-3 zeta (YWHAZ Antibody Abstract)
Background 14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ
UniProt P63104
Pathways Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Storage -20 °C
Storage Comment Store at -20°C / 1 year
Expiry Date 12 months
Images
Image no. 1 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (pSer58) antibody (ABIN1574324) Western blot analysis of extracts from NIH/3T3 cells treated with UV (30mins), using ...
Image no. 2 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (pSer58) antibody (ABIN1574324) PMA + -Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins)...
Image no. 3 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (pSer58) antibody (ABIN1574324) P-peptide - +Immunohistochemical analysis of paraffin-embedded human breast carcinoma...