CA2 antibody (Biotin)

Catalog No. ABIN1607715

Supplier Product No.: 200-406-136-0100

Product Details anti-CA2 Antibody (Biotin)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-406-136-0100

Supplier: Rockland

Purpose: Carbonic Anhydrase II Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes].

Characteristics: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody biotin Conjugation, biotin conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II

Purification: Anti-Carbonic Anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Carbonic Anhydrase II [Human Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Carbonic Anhydrase II Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: CA2 (CA2 Products)

Background: Background: Anti-Carbonic Anhydrase II antibody is necessary for bone resorption and osteoclast differentiation and is involved in the reversible hydration of carbon dioxide. It plays a role in the modulating of fluid secretion into the anterior chamber of the eye. Belonging to the alpha-carbonic anhydrase family, Anti-Carbonic Anhydrase II antibody is ideal for investigators involved in the areas of Cardiovascular and Cell Biology research.

Gene ID: 760

NCBI Accession: NP_000058

UniProt: P00918