Albumin antibody (HRP)

Catalog No. ABIN1607852

Supplier Product No.: 201-4333-0100

Product Details anti-Albumin Antibody (HRP)

Target: Albumin (ALB)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 201-4333-0100

Supplier: Rockland

Purpose: Bovine Serum Albumin Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum].

Characteristics: Synonyms: rabbit anti-bovine albumin peroxidase conjugation, rabbit anti-bovine albumin HRP conjugation, rabbit anti-BSA HRP conjugated antibody, rabbit anti-bovine serum albumin peroxidase conjugated antibody, ALB antibody, Albumin antibody, HRP conjugated Anti-Bovine serum albumin antibody

Purification: Anti-BOVINE ALBUMIN (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Albumin (Bovine Serum)

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:250 - 1:1,000

Application Note: Anti-BOVINE ALBUMIN Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. It is also used as a nutrient in cell and microbial culture. Specific conditions should be optimized by user.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: ALB (ALB Products)

Background: Background: Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard. BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. Anti-Bovine albumin antibody is ideal for investigators involved in serum protein component research.

Gene ID: 280717

UniProt: P02769

Pathways: Lipid Metabolism