RCHY1 antibody

Catalog No. ABIN1724723

Product Details anti-RCHY1 Antibody

Target: RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RCHY1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Pirh2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human Pirh2 expressed in E. Coli.

Clone: 1H10

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-RCHY1 antibody (ABIN1724723)

Duan, Gao, Wu, Zhang, Otterson, Villalona-Calero: "Differential response between the p53 ubiquitin-protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells." in: Experimental cell research, Vol. 312, Issue 17, pp. 3370-8, (2006) (PubMed).

Duan, Gao, Druhan, Zhu, Morrison, Otterson, Villalona-Calero: "Expression of Pirh2, a newly identified ubiquitin protein ligase, in lung cancer." in: Journal of the National Cancer Institute, Vol. 96, Issue 22, pp. 1718-21, (2004) (PubMed).

Target Details for RCHY1

Target: RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Alternative Name: Pirh2 (RCHY1 Products)

Background:

Description: Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.

Aliases: ARNIP, CHIMP, RNF199, RCHY1

Molecular Weight: 30kDa, 60kDa (homodimer)

Gene ID: 25898

HGNC: 25898

UniProt: Q96PM5