GABRA2 antibody (AA 29-37)

Catalog No. ABIN1742454

Product Details anti-GABRA2 Antibody

Target: GABRA2 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 2 (GABRA2))

Binding Specificity: AA 29-37

Reactivity: Human, Mouse, Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This GABRA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for GABA-A receptor alpha2.

Purification: antiserum

Immunogen: Synthetic peptide (aa 29-37 of rat GABA-A receptor alpha2 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet

Comment:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-GABRA2 antibody (ABIN1742454)

Wefelmeyer, Cattaert, Burrone: "Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 31, pp. 9757-62, (2015) (PubMed).

Herrera-Molina, Sarto-Jackson, Montenegro-Venegas, Heine, Smalla, Seidenbecher, Beesley, Gundelfinger, Montag: "Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65." in: The Journal of biological chemistry, Vol. 289, Issue 13, pp. 8973-88, (2014) (PubMed).

Target Details for GABRA2

Target: GABRA2 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 2 (GABRA2))

Alternative Name: GABA-A Receptor Alpha2 (GABRA2 Products)

Background: Synonyms: GABA(A) R alpha2