GABRA6 antibody (AA 20-30)

Catalog No. ABIN1742463

Product Details anti-GABRA6 Antibody

Target: GABRA6 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6))

Binding Specificity: AA 20-30

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for GABA-A receptor alpha6.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 20-30 of rat GABA-A receptor alpha6 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500

Comment:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-GABRA6 antibody (ABIN1742463)

Joensuu, Tegelberg, Reinmaa, Segerstråle, Hakala, Pehkonen, Korpi, Tyynelä, Taira, Hovatta, Kopra, Lehesjoki: "Gene expression alterations in the cerebellum and granule neurons of Cstb(-/-) mouse are associated with early synaptic changes and inflammation." in: PLoS ONE, Vol. 9, Issue 2, pp. e89321, (2014) (PubMed).

Target Details for GABRA6

Target: GABRA6 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6))

Alternative Name: GABA-A Receptor Alpha6 (GABRA6 Products)

Background: Synonyms: GABA(A) R alpha6

Pathways: Synaptic Membrane