Resistin antibody

Catalog No. ABIN181156

Product Details anti-Resistin Antibody

Target: Resistin (RETN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Resistin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects Human Resistin. Other species not tested.

Characteristics: Synonyms: FIZZ3, HXCP1, RSTN, ADSF, Adipose tissue-specific secretory factor,C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-richsecreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3

Purification: Purified from cell culture by Protein A Affinity Chromatography

Immunogen: Highly pure (>98%) recombinant Human Resistin

Isotype: IgG2b

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 200 pg/mL of recombinant human Resistin when used withBiotin anti-human Resistin antibody (cat. PP1214B) as the detection antibody at aconcentration of at least 0.5-1.0 μg/mL. Western Blot: To detect Human Resistin by Western Blot analysis this antibody can beused at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane,under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Resistin

Target: Resistin (RETN)

Alternative Name: Resistin (RETN Products)

Background: Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.Synonyms: ADSF, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3, HXCP1, RETN, RSTN

Gene ID: 56729

UniProt: Q9HD89

Pathways: Feeding Behaviour, Smooth Muscle Cell Migration