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CD40 Ligand antibody (Biotin)

CD40LG Reactivity: Human WB, EIA Host: Goat Polyclonal Biotin
Catalog No. ABIN181577
  • Target See all CD40 Ligand (CD40LG) Antibodies
    CD40 Ligand (CD40LG)
    Reactivity
    • 145
    • 76
    • 34
    • 9
    • 8
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 93
    • 65
    • 21
    • 10
    • 10
    • 2
    Goat
    Clonality
    • 110
    • 86
    • 4
    Polyclonal
    Conjugate
    • 106
    • 23
    • 19
    • 14
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD40 Ligand antibody is conjugated to Biotin
    Application
    • 105
    • 98
    • 54
    • 50
    • 39
    • 29
    • 21
    • 17
    • 16
    • 13
    • 13
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    • 6
    • 4
    • 4
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    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects Human CD154 / sCD40L.
    Purification
    Affinity Chromatography
    Immunogen
    Highly pure (> 98%) E.coli derived recombinant Human sCD40L
    Top Product
    Discover our top product CD40LG Primary Antibody
  • Application Notes
    ELISA: Direct: To detect hsCD40L by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40L. Sandwich: To detect hsCD40L by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumansCD40L as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hsCD40L. Western Blot: To detect hsCD40L by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
    Buffer
    PBS, pH 7.2
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the lyophilized antibody at -20 °C. Following reconstitution the antibody it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
    Expiry Date
    6 months
  • Target
    CD40 Ligand (CD40LG)
    Alternative Name
    CD154 / CD40L (CD40LG Products)
    Background
    CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
    Gene ID
    959
    NCBI Accession
    NP_000065
    UniProt
    P29965
    Pathways
    NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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