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Notch1 antibody (Notch 1) Primary Antibody

NOTCH1 Reactivity: Human, Mouse FACS, IHC (fro), WB Host: Mouse Monoclonal mN1A unconjugated
Catalog No. ABIN181989
$489.50
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Available
  • Target
    Notch1 (NOTCH1)
    Reactivity
    • 134
    • 102
    • 63
    • 15
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 108
    • 43
    • 8
    • 6
    • 5
    • 3
    • 1
    Mouse
    Clonality
    • 112
    • 58
    • 2
    Monoclonal
    Conjugate
    • 88
    • 13
    • 9
    • 9
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This Notch1 antibody is un-conjugated
    Application
    • 88
    • 67
    • 59
    • 42
    • 27
    • 26
    • 20
    • 20
    • 16
    • 14
    • 10
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
    Specificity
    This antibody is specific for Notch1, a single transmembrane receptor which is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow.
    Purification
    Affinity Chromatography on Protein G
    Immunogen
    Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    Clone
    MN1A
    Isotype
    IgG1
  • Application Notes
    Western Blot. Flow Cytometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections (1/50-1/200).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservataive.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Notch1 (NOTCH1)
    Alternative Name
    NOTCH1 (NOTCH1 Antibody Abstract)
    Synonyms
    notch1, NOTCH1, hn1, tan1, notch, xotch, xnotch, notch-1, xnotch1, TAN1, hN1, 9930111A19Rik, Mis6, N1, Tan1, lin-12, NOTCH, Xotch, notch1-a, notch 1, fibropellin-1, Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, notch 1 S homeolog, notch1, LOC100631526, NOTCH1, Notch1, notch1.S
    Background
    The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoieisis, myelopoiesis, and neurogenesis.Synonyms: Neurogenic locus notch homolog protein 1, Notch 1, TAN1, hN1
    Gene ID
    4851
    NCBI Accession
    NP_060087
    UniProt
    P46531
    Pathways
    Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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