ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila)) (Internal Region)

Details for Product anti-ROBO1 Antibody No. ABIN185537
Antigen
Epitope
Internal Region
8
7
5
4
2
2
2
2
1
1
1
1
Reactivity
Human
59
24
24
4
1
Host
Goat
46
10
8
Clonality
Polyclonal
Conjugate
This ROBO1 antibody is un-conjugated
3
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
53
37
9
3
2
1
1
1
1
Options
Purpose ROBO1 / DUTT1 (Internal)
Immunogen Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1, NP_598334.1, NP_001139316.1, NP_001139317.1.
Blocking Peptide Blocking peptide for this product available: ABIN369106
Sequence GDVDLSNKIN EMK
Isotype IgG
Specificity Not expected to cross-react with ROBO2 or ROBO3.
Predicted Reactivity Human, Mouse, Rat, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name ROBO1 (ROBO1 Antibody Abstract)
Background ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
Gene ID 6091
NCBI Accession NP_002932, NP_598334
Pathways Positive Regulation of Endopeptidase Activity
Application Notes

DS WB Results: An anonymous source observed an approx 250 kDa band in Human Endothelial cell lysates at 1 μg/mL antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.

DS IHC Results: Paraffin embedded Human Liver. Recommended concentration: 2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment

Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (Internal Region) antibody (ABIN185537) ABIN185537 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrie...
Image no. 2 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (Internal Region) antibody (ABIN185537) ABIN185537 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabi...
Image no. 3 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (Internal Region) antibody (ABIN185537) ABIN185537 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line),...
Image no. 4 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (Internal Region) antibody (ABIN185537) ABIN185537 (1µg/ml) staining of HUVEC lysate (35µg protein in RIPA buffer). Detected ...