ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila)) (Internal Region) Primary Antibody
ROBO1
Reactivity: Human
ELISA, FACS, IF, IHC, WB
Host: Goat
Polyclonal
unconjugated
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Catalog No. ABIN185537
$481.84
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100 μg
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-
- Target
- Binding Specificity
- Internal Region
- Reactivity
- Human
- Host
- Goat
- Clonality
- Polyclonal
- Conjugate
- This ROBO1 antibody is un-conjugated
- Application
- ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Purpose
- ROBO1 / DUTT1 (Internal)
- Sequence
- GDVDLSNKIN EMK
- Specificity
- Not expected to cross-react with ROBO2 or ROBO3.
- Predicted Reactivity
- Human, Mouse, Rat, Dog, Cow
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1, NP_598334.1, NP_001139316.1, NP_001139317.1.
- Isotype
- IgG
-
-
- Application Notes
DS WB Results: An anonymous source observed an approx 250 kDa band in Human Endothelial cell lysates at 1 μg/mL antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.
DS IHC Results: Paraffin embedded Human Liver. Recommended concentration: 2 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:8000.
- Comment
Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- Alternative Name
- ROBO1 (ROBO1 Antibody Abstract)
- Synonyms
- DUTT1, SAX3, AW494633, AW742721, Gm310, ROBO1, dutt1, roundabout, sax3, MGC122264, roundabout-1, roundabout guidance receptor 1, roundabout, axon guidance receptor, homolog 1 (Drosophila), roundabout guidance receptor 1 L homeolog, ROBO1, Robo1, robo1, robo1.L
- Background
- ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
- Gene ID
- 6091
- NCBI Accession
- NP_002932, NP_598334
- Pathways
- Positive Regulation of Endopeptidase Activity
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