ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila)) (Internal Region)

Catalog No. ABIN185537

Product Details anti-ROBO1 Antibody

Target: ROBO1

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Binding Specificity: Internal Region

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• Internal Region 8
• AA 1632-1644 4
• AA 29-143 4
• AA 491-589 3
• AA 1097-1130 2
• C-Term 2
• AA 1275-1325 1
• AA 19-560 1
• AA 264-897 1
• AA 491-590 1
• AA 730-810 1
• Center 1

Reactivity: Human

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• Human 34
• Mouse 15
• Rat 14
• Dog 3
• Rabbit 1

Host: Goat

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• Rabbit 24
• Goat 7
• Mouse 6

Clonality: Polyclonal

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• Polyclonal 32
• Monoclonal 5

Conjugate: This ROBO1 antibody is un-conjugated

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• Un-conjugated 21
• Biotin 3
• FITC 2
• HRP 2
• APC 1
• Alexa Fluor 488 1
• Alexa Fluor 555 1
• Alexa Fluor 594 1
• Alexa Fluor 647 1
• Alexa Fluor 680 1
• Alexa Fluor 750 1
• Alkaline Phosphatase (AP) 1
• PE 1

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

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• Western Blotting (WB) 28
• ELISA 23
• Immunohistochemistry (IHC) 5
• Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2
• Immunoprecipitation (IP) 2
• Enzyme Immunoassay (EIA) 1
• Flow Cytometry (FACS) 1
• Immunofluorescence (IF) 1
• Immunofluorescence (fixed cells) (IF/ICC) 1

Purpose: ROBO1 / DUTT1 (Internal)

Sequence: GDVDLSNKIN EMK

Specificity: Not expected to cross-react with ROBO2 or ROBO3.

Predicted Reactivity: Human, Mouse, Rat, Dog, Cow

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1, NP_598334.1, NP_001139316.1, NP_001139317.1.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: An anonymous source observed an approx 250 kDa band in Human Endothelial cell lysates at 1 μg/mL antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.

DS IHC Results: Paraffin embedded Human Liver. Recommended concentration: 2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ROBO1

Target: ROBO1

Alternative Name: ROBO1 (ROBO1 Antibody Abstract)

Synonyms: DUTT1, SAX3, AW494633, AW742721, Gm310, ROBO1, dutt1, roundabout, sax3, MGC122264, roundabout-1, roundabout guidance receptor 1, roundabout, axon guidance receptor, homolog 1 (Drosophila), roundabout guidance receptor 1 L homeolog, ROBO1, Robo1, robo1, robo1.L

Background: ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277

Gene ID: 6091

NCBI Accession: NP_002932, NP_598334

Pathways: Positive Regulation of Endopeptidase Activity