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ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1) (Internal Region) Primary Antibody

Verified ROBO1 Reactivity: Human ELISA, FACS, IF, IHC, WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN185537
$481.84
Plus shipping costs $45.00
100 μg
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  • Target
    ROBO1
    Binding Specificity
    • 8
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 32
    • 14
    • 13
    • 2
    • 1
    Human
    Host
    • 22
    • 7
    • 6
    Goat
    Clonality
    • 30
    • 5
    Polyclonal
    Conjugate
    • 19
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ROBO1 antibody is un-conjugated
    Application
    • 26
    • 23
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
    Purpose
    ROBO1 / DUTT1 (Internal)
    Sequence
    GDVDLSNKIN EMK
    Specificity
    Not expected to cross-react with ROBO2 or ROBO3.
    Predicted Reactivity
    Human, Mouse, Rat, Dog, Cow
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1, NP_598334.1, NP_001139316.1, NP_001139317.1.
    Isotype
    IgG
  • Application Notes

    DS WB Results: An anonymous source observed an approx 250 kDa band in Human Endothelial cell lysates at 1 μg/mL antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.

    DS IHC Results: Paraffin embedded Human Liver. Recommended concentration: 2 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    ROBO1
    Alternative Name
    ROBO1 (ROBO1 Antibody Abstract)
    Synonyms
    DUTT1, SAX3, AW494633, AW742721, Gm310, ROBO1, dutt1, roundabout, sax3, MGC122264, roundabout-1, roundabout guidance receptor 1, roundabout, axon guidance receptor, homolog 1 (Drosophila), roundabout guidance receptor 1 L homeolog, ROBO1, Robo1, robo1, robo1.L
    Background
    ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
    Gene ID
    6091
    NCBI Accession
    NP_002932, NP_598334
    Pathways
    Positive Regulation of Endopeptidase Activity
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