ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila)) (Internal Region)

Catalog No. ABIN185724

Product Details anti-ROBO1 Antibody

Target: ROBO1

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Binding Specificity: Internal Region

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• Internal Region 8
• AA 1632-1644 4
• AA 29-143 4
• AA 491-589 3
• AA 1097-1130 2
• C-Term 2
• AA 1275-1325 1
• AA 19-560 1
• AA 264-897 1
• AA 491-590 1
• AA 730-810 1
• Center 1

Reactivity: Mouse

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• Human 35
• Mouse 14
• Rat 14
• Dog 3
• Rabbit 1

Host: Goat

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• Rabbit 24
• Goat 7
• Mouse 6

Clonality: Polyclonal

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• Polyclonal 32
• Monoclonal 5

Conjugate: This ROBO1 antibody is un-conjugated

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• Un-conjugated 21
• Biotin 3
• FITC 2
• HRP 2
• APC 1
• Alexa Fluor 488 1
• Alexa Fluor 555 1
• Alexa Fluor 594 1
• Alexa Fluor 647 1
• Alexa Fluor 680 1
• Alexa Fluor 750 1
• Alkaline Phosphatase (AP) 1
• PE 1

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

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• Western Blotting (WB) 28
• ELISA 23
• Immunohistochemistry (IHC) 5
• Flow Cytometry (FACS) 2
• Immunofluorescence (IF) 2
• Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2
• Immunoprecipitation (IP) 2
• Enzyme Immunoassay (EIA) 1
• Immunofluorescence (fixed cells) (IF/ICC) 1

Purpose: Robo1 / DUTT1 (mouse)

Sequence: QKARPAKKQK HQ

Predicted Reactivity: Mouse, Rat, Dog

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QKARPAKKQKHQ, from the internal region of the protein sequence according to NP_062286.2.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 200 kDa band observed in fetal Mouse Brain lysates (calculated MW of 176 kDa according to NP_062286.2). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Mouse Testis. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ROBO1

Target: ROBO1

Alternative Name: Robo1 (ROBO1 Antibody Abstract)

Synonyms: DUTT1, SAX3, AW494633, AW742721, Gm310, ROBO1, dutt1, roundabout, sax3, MGC122264, roundabout-1, roundabout guidance receptor 1, roundabout, axon guidance receptor, homolog 1 (Drosophila), roundabout guidance receptor 1 L homeolog, ROBO1, Robo1, robo1, robo1.L

Background: Robo1, roundabout homolog 1 (Drosophila) , AW494633, AW742721, DUTT1 , roundabout homolog 1

NCBI Accession: NP_062286

Pathways: Positive Regulation of Endopeptidase Activity