ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila)) (Internal Region)

Details for Product anti-ROBO1 Antibody No. ABIN185724
Antigen
Epitope
Internal Region
8
7
5
4
2
2
2
2
1
1
1
1
Reactivity
Mouse (Murine)
60
24
23
4
1
Host
Goat
46
10
8
Clonality
Polyclonal
Conjugate
This ROBO1 antibody is un-conjugated
3
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
53
37
9
3
2
2
2
1
1
Options
Purpose Robo1 / DUTT1 (mouse)
Immunogen Peptide with sequence C-QKARPAKKQKHQ, from the internal region of the protein sequence according to NP_062286.2.
Blocking Peptide Blocking peptide for this product available: ABIN369648
Sequence QKARPAKKQK HQ
Isotype IgG
Predicted Reactivity Mouse, Rat, Dog
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name Robo1 (ROBO1 Antibody Abstract)
Background Robo1, roundabout homolog 1 (Drosophila) , AW494633, AW742721, DUTT1 , roundabout homolog 1
NCBI Accession NP_062286
Pathways Positive Regulation of Endopeptidase Activity
Application Notes

DS WB Results: Approx 200 kDa band observed in fetal Mouse Brain lysates (calculated MW of 176 kDa according to NP_062286.2). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Mouse Testis. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (Internal Region) antibody (ABIN185724) ABIN185724 (5µg/ml) staining of paraffin embedded Mouse Testis. Steamed antigen retri...
Image no. 2 for anti-Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) (Internal Region) antibody (ABIN185724) ABIN185724 (0.05µg/ml) staining of fetal Mouse Brain lysate (35µg protein in RIPA buf...