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ROBO1 antibody (Roundabout, Axon Guidance Receptor, Homolog 1) (Internal Region) Primary Antibody

Verified ROBO1 Reactivity: Mouse ELISA, IHC, WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN185724
$451.79
Plus shipping costs $45.00
100 μg
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  • Target See all ROBO1 Antibodies
    ROBO1
    Binding Specificity
    • 8
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 33
    • 13
    • 13
    • 2
    • 1
    Mouse
    Host
    • 22
    • 7
    • 6
    Goat
    Clonality
    • 30
    • 5
    Polyclonal
    Conjugate
    • 19
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ROBO1 antibody is un-conjugated
    Application
    • 26
    • 23
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Purpose
    Robo1 / DUTT1 (mouse)
    Sequence
    QKARPAKKQK HQ
    Predicted Reactivity
    Mouse, Rat, Dog
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QKARPAKKQKHQ, from the internal region of the protein sequence according to NP_062286.2.
    Isotype
    IgG
  • Application Notes

    DS WB Results: Approx 200 kDa band observed in fetal Mouse Brain lysates (calculated MW of 176 kDa according to NP_062286.2). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.

    DS IHC Results: Paraffin embedded Mouse Testis. Recommended concentration: 5 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    ROBO1
    Alternative Name
    Robo1 (ROBO1 Products)
    Synonyms
    DUTT1, SAX3, AW494633, AW742721, Gm310, ROBO1, dutt1, roundabout, sax3, MGC122264, roundabout-1, roundabout guidance receptor 1, roundabout, axon guidance receptor, homolog 1 (Drosophila), roundabout guidance receptor 1 L homeolog, ROBO1, Robo1, robo1, robo1.L
    Background
    Robo1, roundabout homolog 1 (Drosophila) , AW494633, AW742721, DUTT1 , roundabout homolog 1
    NCBI Accession
    NP_062286
    Pathways
    Positive Regulation of Endopeptidase Activity
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