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IL5 antibody (Interleukin 5) (AA 20-134) Primary Antibody

IL5 Reactivity: Human ICC, IHC, IP, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1868680
$221.00
Plus shipping costs $45.00
100 μL ABIN1868680
100 μL ABIN1868680
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  • Target
    IL5
    Binding Specificity
    • 14
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 20-134
    Reactivity
    • 85
    • 56
    • 21
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 68
    • 37
    • 14
    • 2
    Rabbit
    Clonality
    • 69
    • 52
    Polyclonal
    Conjugate
    • 75
    • 17
    • 6
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL5 antibody is un-conjugated
    Application
    • 71
    • 59
    • 24
    • 19
    • 18
    • 13
    • 10
    • 9
    • 7
    • 7
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
    Purpose
    Polyclonal Antibody to Interleukin 5 (IL5)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against IL5. It has been selected for its ability to recognize IL5 in immunohistochemical staining and western blotting.
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Interleukin 5 (IL5) corresdonding to Ile20~Ser134 with N-terminal His Tag
    Isotype
    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    Immunohistochemistry: 5-20 μg/mL

    Immunocytochemistry: 5-20 μg/mL

    Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), ProClin
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    IL5
    Alternative Name
    Interleukin 5 (IL5 Antibody Abstract)
    Synonyms
    EDF, IL-5, TRF, Il-5, IL5, interleukin 5, IL5, Il5
    Background
    E-CSF, ESGF, EDF, TRF, Colony-Stimulating Factor,Eosinophil, T-Cell replacing Factor, B Cell Differentiation Factor I, Eosinophil Differentiation Factor
    Pathways
    JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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