DIO2 antibody (Internal Region)

Catalog No. ABIN190862

Product Details anti-DIO2 Antibody

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DIO2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: DIO2

Sequence: EVKKHQNQED RC

Specificity: This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence EVKKHQNQEDRC, from the internal region of the protein sequence according to NP_000784.2, NP_054644.1, NP_001007024.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3 μg/mL. Data obtained from customer.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentratio

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-DIO2 antibody (ABIN190862)

Ma, Brouwer, Wesseling, Raj, van der Want, Boddeke, Balasubramaniyan, Copray: "Multipotent stem cell factor UGS148 is a marker for tanycytes in the adult hypothalamus." in: Molecular and cellular neurosciences, Vol. 65, pp. 21-30, (2015) (PubMed).

Target Details for DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Alternative Name: DIO2 (DIO2 Products)

Background: DIO2, deiodinase, iodothyronine, type II , 5DII, D2, SelY, TXDI2 , thyroxine deiodinase, type II, type 2 iodothyronine deiodinase, type II iodothyronine deiodinaseN: type-II 5'deiodinase

Gene ID: 1734, 13371, 65162

NCBI Accession: NP_000784, NP_054644, NP_001007024

Pathways: Hormone Transport, Hormone Activity