CRHR1 antibody (AA 250-263)

Catalog No. ABIN190893

Product Details anti-CRHR1 Antibody

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Binding Specificity: AA 250-263

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CRHR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: CRHR1 / CRF-R (aa 250 - 263)

Sequence: KLYYDNEKCW FGKR

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Sheep

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence KLYYDNEKCWFGKR, from the internal region of the protein sequence according to NP_004373.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: This antibody has been successfully used in WB on Mouse: Hunt AJ Jr et al, Brain Struct Funct. 2018 Jul,223(6):2685-2698. PMID: 29569009.

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm and vesicles of MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-CRHR1 antibody (ABIN190893)

Hunt, Dasgupta, Rajamanickam, Jiang, Beierlein, Chan, Justice: "Paraventricular hypothalamic and amygdalar CRF neurons synapse in the external globus pallidus." in: Brain structure & function, Vol. 223, Issue 6, pp. 2685-2698, (2019) (PubMed).

Target Details for CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Alternative Name: CRHR1 (CRHR1 Products)

Background: CRHR1, CRF-R, corticotropin releasing hormone receptor 1 , CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f , corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor

Gene ID: 1394, 12921, 58959

NCBI Accession: NP_004373

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity