COL7A1 antibody (AA 97-200, AA 479-578, AA 757-967)

Catalog No. ABIN191494

Product Details anti-COL7A1 Antibody

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Binding Specificity: AA 97-200, AA 479-578, AA 757-967

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL7A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Murine type VII Collagen (aa 757-967). Cross reacts with Human type VII Collagen. Not tested for reactivity with type VII Collagen from other species.

Purification: Protein G Chromatography

Immunogen: Recombinant Murine type VII Collagen (mixture of fragments aa 97-200, aa 479-578, aa 757-967) GST fusion protein. Genename: COL7A1

Application Details

Application Notes: ELISA (1/5.000). Western blot (1/500). Immunocytochemistry on Frozen Sections (1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in aqua bidest to a concentration of 1 mg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at -20 °C.

Target Details for COL7A1

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Alternative Name: Collagen Type VII (COL7A1 Products)

Background: Type VII Collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.Synonyms: COL7A1, LC collagen, Long-chain collagen

Gene ID: 12836

NCBI Accession: NP_031764

UniProt: Q63870