Proliferation Marker antibody

Catalog No. ABIN1959948

Product Details

Target: Proliferation Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: A 14 -16 kD protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.

Purification: Protein A purified

Immunogen: Spleen cells of a human patient with hairy cell leukemia.

Type of Immunogen: Cells

Clone: IPO-38

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IHC, IHC-Fr, IHC-P (10 μg/mL), IP, WB (1:500 - 1:2000)

Usage: This antibody is suitable for ELISA, immunoprecipitation, western blotting and immunohistochemistry (frozen and formal in/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-1 00 permeabilized). An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. Tonsilar tissue is typically used as a positive control.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.5 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details

Target: Proliferation Marker