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HSP27 antibody (Heat Shock 27kDa Protein 1) (Ser15) Primary Antibody

HSPB1 Reactivity: Human, Mouse, Rat IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN197345
$350.90
Plus shipping costs $45.00
50 μL ABIN197345
50 μL ABIN197345
local_shipping Shipping to: United States
Available
  • Target
    HSP27 (HSPB1)
    Binding Specificity
    • 44
    • 40
    • 31
    • 16
    • 13
    • 12
    • 11
    • 9
    • 9
    • 8
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser15
    Reactivity
    • 352
    • 147
    • 137
    • 46
    • 43
    • 34
    • 27
    • 27
    • 22
    • 19
    • 14
    • 11
    • 9
    • 7
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 257
    • 101
    • 1
    Rabbit
    Clonality
    • 238
    • 120
    Polyclonal
    Conjugate
    • 168
    • 27
    • 18
    • 15
    • 14
    • 10
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HSP27 antibody is un-conjugated
    Application
    • 260
    • 126
    • 120
    • 111
    • 86
    • 61
    • 59
    • 57
    • 40
    • 30
    • 21
    • 21
    • 19
    • 9
    • 9
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. HSP27 antibody AP02671PU detects endogenous levels of total HSP27 protein.
    Purification
    Immunoaffinity chromatography.
    Immunogen
    The antiserum was produced against synthesized non-phosphopeptide derived from human HSP27 around the phosphorylation site of serine 15 (G-P-Sp-W-D).
  • Application Notes
    Western blot (1: 500-1: 1000)Immunohistochemistry (1: 50-1: 100)
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C.
  • Target
    HSP27 (HSPB1)
    Alternative Name
    HSPB1 / HSP27 (HSPB1 Antibody Abstract)
    Synonyms
    CMT2F, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, 27kDa, Hsp27, cb153, cb660, hsp1, hsp25, hsp27, id:ibd2821, sb:cb660, zgc:103437, HSPB1, cmt2f, hs.76067, hsp28, heat shock protein family B (small) member 1, heat shock protein 1, heat shock protein, alpha-crystallin-related, 1, heat shock protein family B (small) member 1 L homeolog, HSPB1, Hspb1, hspb1, hspb1.L
    Background
    Hsp27, also referred to as the Estrogen regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of Hsp27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
    Gene ID
    3315
    NCBI Accession
    NP_001531
    UniProt
    P04792
    Pathways
    MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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