Transferrin Receptor antibody (FITC)

Catalog No. ABIN206251

Product Details anti-Transferrin Receptor Antibody (FITC)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues. The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialized proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis. An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer's, Parkinson's, Huntington's and Multiple Sclerosis. Is reported as suitable for use in immunoelectron microscopy. OX-26 detects a band of ~95kD in Western blotting under reducing conditions and ~195kD under non-reducing conditions reflecting it's homodimeric structure.

Purification: Protein G purified

Immunogen: PHA activated rat lymphocytes.

Type of Immunogen: Cells

Clone: OX-26

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1), IHC, IHC-Fr

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C or -20°C if preferred. Product should be stored undiluted. Storage in frost free freezers is not recommended. This Protect from light. Avoid freeze-thaw cycles.

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / Transferrin Receptor (TFRC Products)

Background: Name/Gene ID: TFRC
Subfamily: Metallopeptidase M28B
Family: Protease

Synonyms: TFRC, CD71, TFR1, TRFR, TFR, TR, CD71 antigen, p90, T9, Transferrin receptor, Transferrin receptor protein 1

Gene ID: 7037

Pathways: Transition Metal Ion Homeostasis