CD69 antibody (CD69) (FITC)

Details for Product anti-CD69 Antibody No. ABIN2144678
Target
Reactivity
Human
106
72
29
7
7
7
5
4
4
2
1
Host
Mouse
57
55
36
9
Clonality (Clone)
Monoclonal ()
Conjugate
This CD69 antibody is conjugated to FITC
21
16
12
11
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
114
44
32
31
27
21
13
9
8
8
5
4
3
3
1
Options
Clone 4AFN50
Isotype IgG1 kappa
Characteristics The 4AFN50 monoclonal antibody reacts with human CD69, also known as very eary activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.
Target
Alternative Name CD69 (CD69 Antibody Abstract)
Application Notes Optimal working dilution should be determined by the investigator.
Assay Procedure
  • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
  • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
  • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
  • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
  • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
  • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
  • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
  • [Cell fixation: 2 % formaldehyde solution]
Restrictions For Research Use only
Format Liquid
Buffer Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Images
Image no. 1 for anti-CD69 (CD69) antibody (FITC) (ABIN2144678) anti-CD69 (CD69) antibody (FITC)