Tamalin/GRASP antibody (N-Term)

Catalog No. ABIN233820

Supplier Product No.: 600-401-988

Product Details anti-Tamalin/GRASP Antibody

Target: Tamalin/GRASP (GRASP) (GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP))

Binding Specificity: N-Term

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tamalin/GRASP antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Supplier Product No.: 600-401-988

Supplier: Rockland

Purpose: Tamalin Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Tamalin protein.

Characteristics: Synonyms: rabbit anti-Tamalin antibody, rabbit anti-Grasp antibody, GRASP 1 antibody, GRP1-associated scaffold protein, GRIP associated protein 1 antibody, GRIP1 associated protein 1 antibody, GRIPAP 1 antibody, General receptor for phosphoinositides 1-associated scaffold protein

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse Tamalin protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting, and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 42 kDa in size corresponding to Tamalin protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: 1 μg

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.03 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Tamalin/GRASP antibody (ABIN233820)

Kimura, Kitano, Nakajima, Nakanishi: "Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 9, Issue 7, pp. 631-40, (2004) (PubMed).

Hirose, Kitano, Nakajima, Moriyoshi, Yanagi, Yamamura, Muto, Jingami, Nakanishi: "Phosphorylation and recruitment of Syk by immunoreceptor tyrosine-based activation motif-based phosphorylation of tamalin." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32308-15, (2004) (PubMed).

Kitano, Nishida, Itsukaichi, Minami, Ogawa, Hirano, Mori, Nakanishi: "Direct interaction and functional coupling between metabotropic glutamate receptor subtype 1 and voltage-sensitive Cav2.1 Ca2+ channel." in: The Journal of biological chemistry, Vol. 278, Issue 27, pp. 25101-8, (2003) (PubMed).

Kitano, Kimura, Yamazaki, Soda, Shigemoto, Nakajima, Nakanishi: "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 4, pp. 1280-9, (2002) (PubMed).

Target Details for Tamalin/GRASP

Target: Tamalin/GRASP (GRASP) (GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP))

Alternative Name: Grasp (GRASP Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Tamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domain-containing protein that interacts with group 1 metabotropic glutamate receptors (mGluRs).  The PDZ domain-containing amino-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1 mGluRs.  The carboxyl-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins.  Tamalin forms a protein complex with group 1 mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins.  It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses.

Gene ID: 56149, 103472019

UniProt: Q9JJA9